Question

design an experiment to detect the presence and amount of acetobacter in wine. explain why you would perform such a test.

Answer 1

PLATE DILUTION METHOD ON AGAR PLATES

AIM

To detect the presence and amount of acetobacter in wine.

METHOD

Acetobacter strains oxidised ethanol to acetic acid and forms carbon dioxide and water. Acetobacter is a gram-negative bacterium and are able to ferment glucose , lactose , maltose ,raffinose, galactose etc. The steps includes for detecting the presence and amount of acetobacter in wine are following ;

. Cultivate Acetobacter aceti on GYC agar at 30 degree celsius for 48 hours.

. Detecting the presence of acetobacter in wine , positive acetobacter is control an amplification plot and melting curve.

. DNA extracted from wine samples incubated for 1 week at 27 degree celsius aerobically.

. The susceptibility of acetobacter varied in different isolates from 10² - 10⁷ CFU ml^-1 .

RESULT

If the test is successful, the result is positive . That is acetobacter can be identified and the number of cells can be determined.

CRITERIA TO CHOOSE THIS TEST - Azatobacter is commonly found in wine . Through this test, presence and number of acetobacter can be identify.