Explain the purpose of simple staining. Why is fixing necessary for most staining procedures. List the...
Homework Answers
Simple staining is used to measure the size, shape and dimension of micro organism. It is the basic staining procedure.
Fixing is necessary to adhere the microorganisms to the slide before staining it. It allows bacteria to take up staining more easily.
Steps in preparing grams staining are
*take a clean dry slide
*smear the bacterial culture and heat fix it.
*put crystal violet and leave for 60 seconds and wash it under tap water.
*add grams iodine and leave it for 60 seconds and wash it under tap water.
*add decolourizing agent and leave for 30 seconds. And wash it under tap water.
*add safranin stain and leave it for 60 seconds and wash under tap water and air dry it.
*observe under microscope.
In gram positive bacteria when crystal violet stain is added the bacteria will take up stain and when grams iodine is added the colour will strongly bind and when decolourising agent is added it does not decolourize easily because peptidoglycan layer is thick and it does not take up safranin so they appear violet colour under microscope.
In gram negative bacteria when crystal violet is added it take up the stain and when grams iodine is added the stain will firmly bind and when decolourizing agent is added it decolourizes and takes up safranin stain because it has thin peptidoglycon layer. And they will appear as pink under microscope.
Grams staining is so useful because it helps in differentiating between gram positive and gram negative micro organisms.
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Explain the purpose of simple staining. Why is fixing necessary for
most staining procedures. List the...
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the...
1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the purpose of each of the following reagents in a differential staining procedure: a. Primary stain b. Counter stain c. Decolorizing agent d. Mordant 3. Why is it important for the counter stain to be a lighter color than the primary stain? 4. You have done gram staining for a gram positive bacteria, but at the end you observed pinkish-red bacteria under microscope, what was...
1. There are other staining procedures beyond simple staining and differential staining. Examples include endospore, acid-fast,...
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list the steps of the gram staining procedure in order and fill in the color of...
list the steps of the gram staining procedure in order and fill in the color of gram positive cells and gram negative cells after each step
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Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. Define acidic and basic dyes. What is the purpose of each? 2. What is the purpose of fixing a slide that is to be stained? 3. Why are specimens to be stained suspended in sterile saline or distilled water? 4. Which of the microscope objectives is most satisfactory for studying bacteria?...
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QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
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What are the possible flagellar arrangements on bacteria? Describe the basic structure of peptidoglycan. What is the difference between gram positive and gram negative cell wall structure? Know the procedure and sequence of performing a gram stain, and what is happening to the bacterial cell after each step. What is the significance of a bacterial cell wall? (why is it so important?) How can hypertonic solutions be used to inhibit bacterial growth? What are the 3 types of motility seen...
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1. Describe several advantages of differential staining procedures compared with simple staining techniques. 2. Give the purpose of each of the following reagents in a differential staining procedure: a. Primary stain b. Counter stain c. Decolorizing agent d. Mordant 3. Why is it important for the counter stain to be a lighter color than the primary stain? 4. You have done gram staining for a gram positive bacteria, but at the end you observed pinkish-red bacteria under microscope, what was...
-Fohandl NO Cont. Lat Exercise 5-Smear Preparation Procedure to prepare a smear(from liquid and solid media) - be able to correctly order all steps. Know which tools to use for making the smear. What is a target circle? Why is important to draw it? Why is it important to completely air dry the smear before heat fixing? What are two most important things that occur in heat fixing? Advantage/disadvantage of heat fixing Exercise 6-Simple Staining Basic -Cationic(+) vs. Acidic Dyes...
Simple Staining Data Interpretation Please type the answers to the following questions below (double spaced) and attach the data collection sheets when you hand in this lab report. 1. Define acidic and basic dyes. What is the purpose of each? 2. What is the purpose of fixing a slide that is to be stained? 3. Why are specimens to be stained suspended in sterile saline or distilled water? 4. Which of the microscope objectives is most satisfactory for studying bacteria?...
QUESTIONS 1) Are acid fast cells gram positive or gram negative? Explain why it might be difficult to determine the Gram reaction of acid fast cells. 2) What is the significance of the mycolic acid layer in the following contexts? A) To bacteria for survival and infection B) During the acid fast staining procedure QUESTIONS 1) What is the difference between a pure culture and a pure colony? Is a streaking for isolation plate a pure culture when your original...
QUESTIONS Predict the effect on Gram-positive and Gram-negative cells of the following mistakes made when performing a Grum stain. Consider cac mistake independently a. Failure to add the iodine. b. Failure to apply the decoloriser. 3 c. Failure to apply the safranin. d. Reversal of crystal violet and safranin stains. Both crystal violet and safranin are basic stains and may be used to do simple stairs on Gram-positive and Gram-negative cells. This being the case, explain how they end up...
5. Why are simple stains able to "stick” to bacteria? Explain the chemical properties of the stain and bacteria surface. 3-Preparation of Bacterial Smears and Simple Stains Introduction Microbial cultures are handled using aseptic technique. Aseptie technique is a set of procedures used to minimize contamination of materials by microorganisms from the environment, and to minimize the spread of cultures around the laboratory. In this experiment, you will be using an inoculating loop to transfer bacteria from a culture tube...
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