You have sequenced a piece of wild-type DNA from mouse. It clearly contains a gene, but you do not know what gene it is. How would you use the cloned wild-type gene to find out its mutant phenotype?
A. knock out
B. overexpression
C. QTL mapping
D. quantitative real-time PCR
Answer: Knock out
Explanation:
You have sequenced a piece of wild-type DNA from mouse. It clearly contains a gene, but...
You have three genes on the same chromosome - A, B and C. Each gene has two alleles in a dominant/recessive relationship. For these genes the homozygous recessive has the mutant phenotype for that trait, the dominant phenotype = wild type for that trait. allele A is dominant to a; phenotype a = mutant for trait a; phenotype A = wild type for trait A allele B is dominant to b; phenotype b = mutant for trait b; phenotype B...
please help me!!!
5.) You have discovered mutants in two new gene mutants in Drosophila. One mutant, curl, has small, curly, nonfunctional wings. The other mutant, big, has enormous eyes. You decide to test if these two new genes are on chromosome #2, so you perform a three-gene linkage testcross using a gene that you know is on chromosome #2, trp, a mutant that cannot make its own tryptophan (and so must get it from its food). In all three...
5.) You have discovered mutants in two new gene mutants in Drosophila. One mutant, curl, has small, curly, nonfunctional wings. The other mutant, big, has enormous eyes. You decide to test if these two new genes are on chromosome #2, so you perform a three-gene linkage testcross using a gene that you know is on chromosome #2, trp, a mutant that cannot make its own tryptophan (and so must get it from its food). In all three cases, these mutant...
5. Consider the wild-type F8 gene. What would happen to the 1)
transcript
(mRNA) sequence and quantity, 2) to the protein (sequence,
quantity, function) and 3) the person’s
overall phenotype if they were homozygous (both copies of the
gene are the same) for a 3 base-pair
deletion in the:
i. First intron
ii. First exon
iii. The promoter
iv. 5’UTR
v. Last exon
6. What environmental factor could affect the phenotype caused
by an F8 null mutation?
Thrombophilia is a...
1) You have two human liver cells (A and B) and you hypothesize that the insulin receptor gene in Cell A has a mutation in exon 1 and Cell B contains the wild type sequence. You extract genomic DNA from each of the cells. Of the following, what would be the most efficient (quick, precise and relatively cheap) way to test your hypothesis. a. Isolate protein from both cells, purify the insulin receptor, and determine the amino acid content. b. Sequence the...
A. Which strain is the wild-type E.coli? Explain how you know
this.
B. Which strain contains the nonsense mutation in the CRP
protein gene? Explain how you know this.
C. Which strain contains the deletion mutation in the lac operon
operator sequence? Explain how you know this.
knowledge about the lac operon system AND knowledge Problem set about the Lac operon (these problems require knowledge about the lac operon system AND about consequences of mutations) You are working with three...
You have a P-element induced mutation that is homozygous lethal. You think that the element might be inserted into the DMAP1 gene. Which of the following techniques can be used to confirm (or not) that DMAP 1 is the gene responsible for the lethality? Rescue using the DMAP1 cDNA ORNAi for DMAP1 Obtain a large deletion on the chromosome and test for failure to complement two of these all of these White eyed flies can be generated in several different...
2. A dominant allele H reduces the number of body bristles that Drosophila flies have, giving rise to a “hairless” phenotype. In the homozygous condition, H is lethal. An independently assorting dominant allele S has no effect on bristle number except in the presence of H, in which case a single dose of S suppresses the hairless phenotype, thus restoring the "hairy" phenotype. However, S also is lethal in the homozygous (S/S) condition. What ratio of hairy to hairless flies...
21) You have this small piece of sequence data from a portion of your favorite gene that was isolated fronm a ladybug cDNA library. The spaces are to help with counting and the part not show in the midle () is 263 bp. 5' tca tta tgg tgt ttg agt aca tga aac acg gag atc tca ac.. agg tgo gaa tgg cag acg gac age aga gtt 3' a) Design forward and reverseprimersfor PCR that will isolate EXACTLY the...
6a) You want to use homologous recombination to generate a mouse that does not express a functional XPC gene (‘Knock-out’ or KO). To do this, you want to delete exon 10 and replace it with a gene that confers resistance to the drug Neomycin (NEO. The usual second selection with tk we discussed in class is not shown here). Using the diagram as a guide: illustrate on the diagram where you expect crossing over to occur and (below the diagram)...