Describe the subunit structure of the lac repressor and relate it to the symmetrical sequence of...
Describe the subunit structure of the lac repressor and relate it to the symmetrical sequence of the lac operator. Compare affinities of the lac repressors for lac operator DNA and nonspecific DNA. Describe the effect
Homework Answers
The repressor bind to a planidromic operator sequence that span 22 base pair with the larger Regularity region of the lac operon. The symmetry of the operator sequence matches a twofold axis of symmetry in arrangement of lac represser subunit.
The lac represser is a DNA binding protein that inhibit the expression of genes coding for protein involved in the metabolism of lactose in bacteria.
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Describe the subunit structure of the lac repressor and relate
it to the symmetrical sequence of...
The lac repressor active site The active site of the lac repressor protein is composed of...
The lac repressor active site The active site of the lac repressor protein is composed of OK Multiple Choice Inces O the protein domain that binds to allolactose. the protein domain that binds to DNA the protein domain that binds to other lac repressors O a glucose binding domain < Prev
The lac operon contains a DNA sequence known as the lac promoter (P or P+ for...
The lac operon contains a DNA sequence known as the lac promoter (P or P+ for wild type; P– for mutant (RNA polymerase does not bind)) that serves as the RNA polymerase binding site. The lac operon also contains a DNA sequence known as the Lac operator (O or O+ for wild type; O– or Oc for mutant (lac repressor cannot bind)) which is the binding site for lac repressor. The lac repressor, a protein, is encoded by the lac...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of...
Gene Regulation Describe the regulation of the lac operon in E. coli. a. the structure of lac operon in E.coli. b. Role of CAP and cAMP the activator binding site c. Role of Lac Repressor and Operator and Inducer d. Role of Looping e. Effect of mutations f. Recessive v dominant mutations g. Complementing v noncomplementing mutations. Describe the regulation of the trp operon in E. coli. Describe at least three kinds of molecular switches involved in gene regulation. What...
Describe how the Lac repressor and the CAP-cAMP complex regulate the expression of the Lac operon...
Describe how the Lac repressor and the CAP-cAMP complex regulate the expression of the Lac operon in E. coli.
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon...
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon is regulated. The descriptions of some of the mutants are listed. For each E. coli mutant determine if the lac operon would be on or off in a lactose-only environment. laci mutant: This mutant produces a super repressor that is not inactivated by allolactose. It will still stick. Operater will be off, lacO mutant: This mutant has an altered operator sequence so that the...
Match the protein listed below with the gene that encodes it or with the DNA sequence...
Match the protein listed below with the gene that encodes it or with the DNA sequence within the lac operon where it binds: lac repressor (encoded by...) [Choose] lac repressor (where it binds...) [Choose ] B-galactosidase [Choose] Lactose permease [Choose] Galactoside transacetylase [Choose] CAP [Choose] lacz lacy lacl Operator CAP site lacA
a) For the lac operon, will the repressor or RNA polymerase be bound to the operon in this situation? Draw what wil...
a) For the lac operon, will the repressor or RNA polymerase be bound to the operon in this situation? Draw what will be happening on the operon below. PROMOTER OPERATOR Lactose Enzyme 1 Lsctose Enzyme2 Lactose Enzyme 3 NO b) Will transcription occur? c) Describe what is happening (with vocabulary words). YES 2. Bobby Joe is fasting today, how will the E. coli in her stomach respond to the lack of Tryptophan? a) For the trp operon, will the repressor...
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site...
Lactose (or rather, its derivative allolactose) can lift the Lac Repressor protein from the Operator site in DNA, thus allowing for expression of the genes coded by the Lac Operon. However, lactose cannot cross the bacterial cell membrane without the help of the Permease protein channel, which is coded by the lacY gene in the Lac Operon. So, it appears that we have a case of "what's first, the chicken or the egg?" - how can lactose enter the cell...
Describe and label the regions on the lac operon. Include the following: regulatory gene, promoter region,...
Describe and label the regions on the lac operon. Include the following: regulatory gene, promoter region, operator, structural genes, and repressor. What happens in the presence of glucose? Lactose?
21. Double helix 22. Repressor protein 23. Adenine 24. Ribosome.
21. Double helix22. Repressor protein23. Adenine24. Ribosome.25. Promoter26. Replication27. RNA Polymerase.28. CodonA. Enzyme that synthesizes RNAB. Organelle where proteins are assembledC. Complementary to either Thymine or UracilD. mRNA sequence that codes for one amino acidE. Shape of double stranded DNAF. Sequence of DNA that controls gene expressionG. binds an operator and stops gene expression in LAC operon by preventing RNA polymerase from binding gene and transcribing. H. Duplication of DNA in 5 phase of Interphase
The lac repressor active site The active site of the lac repressor protein is composed of OK Multiple Choice Inces O the protein domain that binds to allolactose. the protein domain that binds to DNA the protein domain that binds to other lac repressors O a glucose binding domain < Prev
5. Jacob, Monod, and Pardee used various E.coli mutants to help determine how the lac operon is regulated. The descriptions of some of the mutants are listed. For each E. coli mutant determine if the lac operon would be on or off in a lactose-only environment. laci mutant: This mutant produces a super repressor that is not inactivated by allolactose. It will still stick. Operater will be off, lacO mutant: This mutant has an altered operator sequence so that the...
Match the protein listed below with the gene that encodes it or with the DNA sequence within the lac operon where it binds: lac repressor (encoded by...) [Choose] lac repressor (where it binds...) [Choose ] B-galactosidase [Choose] Lactose permease [Choose] Galactoside transacetylase [Choose] CAP [Choose] lacz lacy lacl Operator CAP site lacA
a) For the lac operon, will the repressor or RNA polymerase be bound to the operon in this situation? Draw what will be happening on the operon below. PROMOTER OPERATOR Lactose Enzyme 1 Lsctose Enzyme2 Lactose Enzyme 3 NO b) Will transcription occur? c) Describe what is happening (with vocabulary words). YES 2. Bobby Joe is fasting today, how will the E. coli in her stomach respond to the lack of Tryptophan? a) For the trp operon, will the repressor...
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