Question

CAN SOMEONE PARAPHRASE OR REWORD THIS PARAGRAPH FOR ME? PLEASE AND THANK YOU!!

Cellular respiration is a process that converts energy from food molecules into the energy of ATP. It involves three stages that occur in the cytoplasm of cells and in the mitochondria. One of the three stages is the citric acid cycle which is a series of reactions that generates reduced electron carriers that transport electrons to the electron transport chain. Since the primary function of the mitochondria is to produce ATP, the citric acid cycle most likely occurs in the mitochondria. To test this hypothesis, the cellular components from cauliflower, namely the nuclei and the mitochondria, were separated using differential centrifugation. The oxidation of succinate to fumarate catalyzed by succinate dehydrogenase is a reaction that is part of the citric acid cycle. This reaction was utilized along with an artificial electron acceptor DCIP. When reduced, DCIP turns from a blue/violet color to a clear solution. DCIP absorbs light most efficiently as 410 nm and the change in absorbance over time was used to show that the citric acid cycle occurs in the mitochondria.

Answer 1

Respiration is a process in which energy is produced from the breaking down of complex food into simpler substances with the production of energy in the form of ATP. The respiration occurs mainly in the three steps first step is glycolysis that occurs in the cytoplasm, transition reaction connects glycolysis to the citric acid cycle, Citric acid cycle and finally the electron transport chain. The citric acid cycle is one of the main steps in the respiratory pathway as it collects the electrons produced in the various reactions by the electron carriers like NADH and FADH2 to be further passed on to the electron transport chain. All these reactions occur in the mitochondria. In order to test the hypothesis that whether the citric acid cycle occurs in mitochondria or nucleus an experiment was done on Cauliflower in which nucleus and mitochondria were separated by differential centrifugation method. One of the primary reaction of Citric acid is chosen for example the oxidation of succinate to fumarate catalyzed by succinate dehydrogenase. For this reaction, DCIP is given which act as an artificial electron acceptor. It accepts the electron and gets reduced. The reduction of DCIP is shown by the change in absorbance of the light as oxidized DCIP and reduced DCIP are of different colours. So when it gets reduced it shows the change in its absorbance, suggesting that electron transfer has occurred in the mitochondria. This actually suggests that the electron transfers do occur in the mitochondria that help in the production of ATP and thus helping to conclude that Citric acid cycle does occur in mitochondria and not in nucleus