Suppose a broth has 436 million bacteria per milliliter. A serial dilution is made using three...
Suppose a broth has 436 million bacteria per milliliter. A serial dilution is made using three bottles of 99 milliliters each.
1mL is transferred from the sample to the first bottle;
1 mL is transferred from the first bottle to the second;
and then 1 mL is transferred from the second bottle to the third.
From the third bottle 0.1 mL is transferred to pour plate.
How many colonies would you expect to find on the plate? Show your work.
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Suppose a broth has 436 million bacteria per milliliter. A
serial dilution is made using three...
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1....
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
A polluted water sample has 2,400,000 bacteria per ml. You transfer 1.0 ml of sample to...
A polluted water sample has 2,400,000 bacteria per ml. You transfer 1.0 ml of sample to a bottle containing 99ml of sterile water. You then take 0.1 ml from this dilution bottle and transfer to a second bottle with 0.9 ml of sterile water. Finally, you transfer 1 ml to a Petri dish with nutrient agar. How many colonies would you expect to find on this plate? Would it be countable?
You have a tube with 5ml of infected blood that has a concentration of bacteria at...
You have a tube with 5ml of infected blood that has a concentration of bacteria at 10(8) cells per ml. You wish to make a serial dilution of the blood so when you take 100 ul of the last dilution and spread it on a Petri dish you will have between 30-300 colonies growing on the plate. You have plenty of empty 1.5 ml microfuge tubes, a bottle of sterile water, a p1000 pipettor (100-1000ul) and tips, for the dilutions,...
Answer the following questions regarding a 10x Serial Dilution experiment using yeast cell cultures from tube...
Answer the following questions regarding a 10x Serial Dilution experiment using yeast cell cultures from tube A. Tube A contains 1 ml of yeast culture. 3.6 ml of sterile water is placed into 4 tubes (B, C, D, and E). 0.4 ml of yeast culture is removed from tube A and put into tube B.. 0.4 ml is transferred from tube B to tube C, and from tube C to tube E. 1.) What is the final volume in tubes...
please help with whatever possible. thank you so much in advance. Name One use of serial...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
I need help answering the following microbiology questions. 1. The three tubes in the image to...
I need help answering the following microbiology
questions.
1. The three tubes in the image to the right contain glucose and the pH indicator phenol red. The middle and left tubes were inoculated with bacteria, and the right tube was left uninoculated as a control. Which of the tubes show evidence of heterolactic fermentation? a. Left tube Gas b. Middle tube c. Right tube d. None of the three tubes Yellow Red Red 2. Which of the three tubes in...
Bacteria #2 is a Bacteria #3 is a III. Calculation: (1) Serial dilution: (3 pts/each) 1. 0.2 mL of sample is added to 0.8 mL of diluent, what is the Dilution? 2. 0.01 mL of sample is added to 0.99 mL of diluent, what is the Dilution? :100 mL 0.2 mL of a sample was added to 0.8 mL of a diluent. Then, 0.1 mL of this first dilution was added to 0.4 mL of a diluent. What is the...
please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
I need help answering the following microbiology
questions.
1. The three tubes in the image to the right contain glucose and the pH indicator phenol red. The middle and left tubes were inoculated with bacteria, and the right tube was left uninoculated as a control. Which of the tubes show evidence of heterolactic fermentation? a. Left tube Gas b. Middle tube c. Right tube d. None of the three tubes Yellow Red Red 2. Which of the three tubes in...
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