Question

You have a protein solution.

You take 5 ml of the solution, and add 25 ml of buffer.

You then add 50 µl of the dilute protein solution to 50 µl of buffer and 2 ml of Coomassie solution.

The resulting absorbance at 595 nm was 0.457.

At the same time, you prepared a standard curve for the same protein using the same Coomassie assay protocol (100 µl of sample + 2 ml of coomassie reagent)

The linear fit to the Coomassie standard curve was:

Y = 0.085 + 0.032x

Where y = absorbance at 595nm and x = µg protein added to the assay.

What is the protein concentration in the original, undiluted protein solution

Answer 1