Question

You perform an in vitro treatment of chicken fibroblast chromatin with DNaseI. Fibroblasts synthesize extracellular matrix and collagen producing the extracellular structural framework animal tissues. The DNA fragments are denatured and immobilized on a filter. You allow for hybridization of single stranded radioactive globin cDNA with the DNA immobilized on the filter, wash away any unhybridized probe, and determine the amount of radioactivity (counts per minute; CPM) bound to the filter compared to a negative control experiment in which you do not perform the in vitro digestion of DNA with DNaseI. You determine that you get 95% of the radioactivity bound to the filter with DNaseI treated chicken fibroblast chromatin compared DNaseI untreated chicken fibroblast chromatin. Which of the following is a conclusion consistent with the data?

A) Ovalbumin genes are NOT expressed in chicken fibroblasts

B) Ovalbumin genes are expressed in chicken fibroblasts

C) Globin genes are expressed in chicken fibroblasts.

D) The chromatin structure around the globin gene is likely compacted and dense in chicken fibroblasts.

E) The chromatin structure around the globin gene is likely open and loose in chicken fibroblasts.

Answer 1