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You have 5ml of electrophoresis buffer. Adjust it to 0.1% Commasie blue (wt/vol)

You have 5ml of electrophoresis buffer. Adjust it to 0.1% Commasie blue (wt/vol)

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Answer #1

given 0.1% Commasie blue (wt/vol) it means that:

100 ml of buffer solution contains = 0.1 gm of Commasie blue

1 ml of buffer solution contains = 0.1/100 gm of Commasie blue

5 ml of buffer solution contains = (0.1/100) x 5 gm of Commasie blue

= 0.005 gm of Commasie blue

so 0.005 g of Commasie blue added to 5 mL of the buffer.

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