What are immortalized cells? Give a practical method to make a cell culture immortal. How can...
What are immortalized cells? Give a practical method to make a cell culture immortal.
How can we differentiate an immortalized cell line from a cancer cell line?
Explain the procedure used to make a chimeric mouse.
Homework Answers
As per our regulations the first question is to be answered. One more is also answered for your benefit.
Ans. 1 Immortalized cells are the group of cells of
multicellular organism which due to mutation, can escape normal
cellular senescence and keep division continuous. Thus they have
ability to grow in vitro for a prolonged period of time. However
mutations required for immortality can be natural or be
intentionally induced for experimental purpose.
The cells are manipulated to circumvent the process of senescence
and become immortal by Introducing a Viral Gene method. This can
override the Cell Cycle and thus can be used to overcome senescence
by hindering function of the biological brakes on proliferative
control.
Ans. 2 Major differences between normal cells and cancerous cells is visible from the chromosomes morphology (karyotype). In oppose to cancerous cells (tumorogenic) an immortalised cell line would normally not proliferate indefinitely but, due to mutation, it evades normal cellular senescence and divides. The cells can therefore be grown for prolonged periods in vitro.
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