calulate the # of generation, generation time and growth rate for the following e coli bactetia...
If E. coli is grown under conditions that maintain a generation time of 80 minutes and the cells are the quickly transferred into a new medium that allows a generation time of 40 minutes? 1. How would that affect DNA polymerase? 2. DNA replication process? 3. DNA division? 4. Will generation time change?
If escheria coli has a generation time of 20 minutes, will you be able to construct a growth curve from the following data ? Initial time 0 absorbance 0.05 cell number per every mL 1.0x107 . Ending Time 20 minutes absorbance 0.07 cell number 1.0x107 ?
Consider a culture of E. coli growing in exponential phase with a generation time of 20 minutes. Beginning with 2 x 106 cells per ml, how long will it take the culture to reach a density of about 3.2 x 107 cells per ml?
3) Use the logarithmic phase of the growth curve to estimate the generation time (g units are hours/generation; also known as the doubling time) of E. coli. How does the generation time that you estimated compare to the published generation times for E. coli growing under optimal conditions? What are some of the possible reasons for differences in the generation times? What are possible sources of error in this exercise? OD600 readings and cell concentration OD600 Readings Cell Concentration (cell/mL)...
E. Coli has a doubling time of 20 minutes under certain growth conditions. Compute the biomass of E. coli (in pounds) that will accumulate when a single cell of E. coli grows exponentially for 50 hours in these conditions, assuming that nutrients are not limiting. Show your work. (An E. Coli cell has a mass of 10-12gram/cell).
If the osmolarity of a growth medium is rapidly doubled, then the E. coli cells growing in that medium are likely to _______________________. compensate by decreasing the osmolarity inside the cell shrink pump Na+ out of the cell convert glucose to starch pump water into the cell
An E. coli culture is grown in a variety of very rich media. With a number of the media you are able to reduce the generation time to 20 mins. No further additions to the media reduce the doubling time below 20 mins. The supply of nutrients and building blocks are not limited. What factors could be limiting the growth rate of the cell?
13. The rate of B-galactosidase synthesis in E. coli is influenced by the bacteria's genotype and its growth conditions. Indicate whether you would expect the rate of β-galactosidase synthesis to increase, decrease, or remain the same after each of the following changes to the growth medium. Explain your answer. a. E. coli cells are cultured in minimal medium containing glycerol as the sole b. E. coli cells are cultured in minimal medium containing glucose as the sole c. E. coli...
please use these results of a growth curve experiment to
answer the following questions
Questions 1) Plot the OD600 readings and cell concentration for each time point on a scatterplot with a line connecting the points. Have the measured data (OD600 and cell concentration) on the y-axis against the time on the x-axis using Excel (or other computer based data/graphing program). It is helpful to set the y-axis on a log scale for the cell concentration measurements. 2) Can you...
3. You inoculate 1 liter of medium with an E. coli culture to an initial cell density of 104 cells/ml. The culture grows exponentially with a generation time of 20 minutes. What is the culture density after 24 hours?