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QUESTION 16 "The following are genotypes of merozygotes of E. coli with various combinations of lac...

QUESTION 16

  1. "The following are genotypes of merozygotes of E. coli with various combinations of lac operon mutations. Determine the phenotype with respect to beta-galactosidase (z), permease (y), and acetylase (a) of each combination as U = uninducible, I = inducible, and C = constitutive.

    i- p+ o+ z+ y+ a-/i+ p+ oc z- y+ a+ "

    CCI

    UUU

    UCC

    III

    UIC

    IUI

    CIU

    ICC
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Answer #1

i- p+ o+ z+ y+ a-/i+ p+ oc z- y+ a+

here in the first operon the functional receptor gene is absent but the other operon has functional repressor gene, repressor produce by the functional repressor in the second operon can inhibit the expression of first operon (i- p+ o+ z+ y+ a-) lactose is an inducer, it can inhibit the lac repressor so lactose can induce the expression of lac operon, in the presence of an inducer the i- p+ o+ z+ y+ a- is expressed, but the other operon has constitutive operator so it cannot be inhibited by the lac repressor so the operon i+ p+ oc z- y+ a+ is expressed always so the genes y+ and a+, which codes for the permease and acetylase are expressed always but the beta-galactosidase produced from the other operon only in the presence of an inducer so the answer is ICC ( beta-galactosidase is inducible, permease and acetylase is constitutive)

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