Utilize mutations to analyze the various control elements of a eukaryotic promoter.
Utilize mutations to analyze the various control elements of a eukaryotic promoter.
What are the four common core promoter elements for eukaryotic genes transcribed by RNA polymerase II? Must all of these elements be present in the promoter for transcription of every gene to occur? Explain
-Which elements are found in a eukaryotic promoter vs. a prokaryotic promoter? -what is the concept of (restriction enzyme produced) DNA fragment separation by gel electrophoresis -what are the steps and process of thermal cycling? -what is the restriction enzyme(s) and how do you know when they leave blunt or sticky ends (ie. XbaI, SmaI, EcoRI, BamHI)? -The lac and trp operons (form a figure showing the operon). - What is RNA silencing involvee (in general, what is RNA interference;...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the DNA immediately upstream of the transcription start site, but expression of the gene can also be affected by control elements. These can be thousands of base pairs upstream of the promoter, grouped in an enhancer. Because the distance and spacing of these control elements make them difficult to identify, scientists begin by deleting sections of DNA that contain possible control elements and measuring the...
Choose the cis-elements. Click on the cis regulatory elements only. Control of eukaryotic gene expression (gene control) can occur at multiple levels from DNA accessibility to post-translational changes to polypeptides/proteins. Silencers, repressors, transcription factors, activators, enhancers, insulators histone acetyltransferases (HATS), histone deacetylases (HDACs) histone remodellers and DNA methyltransferases all play a role in gene control.
promoter mutations are: trans-dominant, cis-dominant, trans-recessive, or cis-recessive? when glucose levels drop in a bacterial cell: cAMP is induced, cAMP is repressed , cGMP is induced or cGMP is repressed? the trp repressor functions to control its own expression as an example of: induction by negative control, induction by positive control, repression by negative control or repression by positive control
The diagram below shows two stretches of DNA in the genome of an
imaginary eukaryotic cell. The top stretch of DNA includes the
fantasin gene, along with its promoter and one of its enhancers.
The bottom stretch of DNA includes the imaginin gene, its promoter,
and one of its enhancers. The slash marks (//) indicate that more
than 1,000 nucleotides separate the promoter and enhancer of each
gene
Which statements about the regulation of transcription
initiation in these genes are...
Mutations in the exons of eukaryotic genes can result in changes in the translated protein. Nonexonic mutations, mutations in regions of a gene outside of the exons, can also alter the rate of transcription and the final protein. In which of the following situations would nonexonic mutations either affect expression of the gene or alter the structure of the final protein? Select all of the statements that apply.
Which of the following correctly describes eukaryotic transcriptional control? a. A transcription factor is a DNA molecule that helps RNA polymerase to bind to the enhancer of a specific gene. b. An enhancer is a protein that encourages gene expression by binding to the DNA. c. The promoter is the region of RNA where DNA polymerase will bind to begin transcription. d. The interaction of multiple transcription factors may be required in order to transcribe a...
Can someone please help me answer these questions. Thank
you!
Eukaryotic transcription signals
a) This drawing shows the placements of the four main sequences of
the eukaryotic core promoter for RNA polymerase II. Identify each
one and give a brief explanation
b) Which sequences are used in a DPE-driven promoter?
c) Which ones are used in a TATA-driven promoter?
d) Please draw and describe the steps as the transcription factors
work with eukaryotic RNA polymerase II to start transcription of...
Analyze how mutations can be inherited or acquired.