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How would the primer be used in identification of Hepatitis C virus? This question regards identification...

How would the primer be used in identification of Hepatitis C virus?

This question regards identification of the Hepatitis C virus through a polymerase chain reaction.

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Polymerase chain reaction (PCR) is a method which is extensively used in molecular biology to make many copies of a specific DNA segment at a large scale within a vry short period of time.

HCV is a RNA virus. For this reson HCV RNA PCR test is used to determine whether the hepatitis C virus (HCV) exists in a person's bloodstream. If the virus is present, the test can also measure the exact amount (titer) in your blood. This amount is known as the viral load.

The primer used in the detection of specific pathogen should be very much accurate and specific, otherwise false positive result could be obtained. In HCV, primers are prepared from the 5' Non Coding region which have been reported to detect HCV virus precisely.

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