During your research project, you have identified the novel protein Golgo1 that appears to localize to...
During your research project, you have identified the novel protein Golgo1 that appears to localize to the Golgi. You would like to demonstrate that Golgo1 indeed localizes to the Golgi apparatus by comparing its localization to a known Golgi marker protein. You decide to perform your study in cells that express a green fluorescent protein (GPF) tagged form of the known Golgi marker protein mannosidase II.
Which of the following antibody combinations would you use for such a co-localization experiment?
A) rabbit-anti-Golgo1, goat anti-rabbit-green fluorochrome
B) rabbit-anti-Golgo1, goat-anti-mouse –green fluorochrome
C) mouse-anti-mannosidase II, goat-anti-mouse-red fluorochrome
D) guinea-pig –anti- Golgo1, goat –anti-guinea pig-red fluorochrome
E) the combinations listed in b and c would both work.
Homework Answers
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ANSWER :-
Option (d) is the correct answer which is , guinea -pig-anti-Golgo1,anti-guinea pig-red fluorochrome . This antibody combination is used for co-localization experiment .
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