Question

QUESTION 1

SNPs contain

variable amino acid substitutions and are highly heterogeneous.

various nucleotides transcribed repeatedly and are homogeneous

. variable base sequences and are variable in the population.

variable numbers of tandem repeats and are highly uniform in the population

. small noteworthy transcribed regions and are lethal genes.

QUESTION 2

Which of the following is a potential use for DNA microarrays?

trap genes that are both active and inactive

isolate genes from eukaryotic cell nuclei

assay protein output from a genomic database

mutate genes of interest

scan a population of nucleic acids for abundance and mutations

QUESTION 3

What enzyme is responsible for generating restriction fragments?

Endonuclease

Polymerase

Ligase

RNase

Phosphatase

Answer 1

1) The answer will be variable base sequences & are variable in the population (third option).

Explanation: SNPs (single nucleotide polymorphisms) is a type of genetic variation that occurs in a single nucleotide base at the specific position of the genome of a population. To consider it as a SNP this variation must be occur at least 1% of the population. It can be of two types- linked & causative. Linked SNPs stay outside gene & causative SNPs stay within gene. Thus SNPs contain variable base sequences & are variable in the population. So, correct answer will be third option. All other given options are wrong.

2) The answer will be scan a population of nucleic acids for abundance and mutations (Last option).

Explanation: DNA microarray is a technique by which we can simultaneously detect the expression of several genes. Thus this technique is useful for scanning nucleic acids of a whole population & search for mutation or abundance of nucleic acids. So, correct answer will be last option.

3) The answer will be endonuclease (First option).

Explanation: Polymerase enzyme synthesize nucleic acids. So we can ignore second option. Ligase helps in the formation of phosphodiester bond & ultimately facilitate the sealing of DNA strand. So we can ignore third option. RNase helps to degrade RNA. So we can ignore fourth option. Phosphatases are a type of hydrolase which with the help of water cleave a phosphoric acid monoester into a phosphate ion and alcohol. So we can ignore last option. Endonucleases are enzymes that cleave phosphodiester bonds within a polynucleotide chain. Restriction endonucleases are restriction enzymes that cleave at specific nucleotide sequence. So, correct answer will be endonuclease (First option).