What would be the effects on DNA replication if mutation of DNA polymerase III caused it...
What would be the effects on DNA replication if mutation of DNA polymerase III caused it to lose each of the following activities: 1. 5' to 3' polymerase activity 2. 3' to 5' exonuclease activity:
Homework Answers
when replication errors become mutation. Incorectly paired nucleotides that still remain following mismatch repair become permanent mutations after the next cell divisions. This is because one mistake are establised, the cells are no longer recognizes them as errors.
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What would be the effects on DNA replication if mutation of DNA
polymerase III caused it...
Describe DNA replication on the leading and lagging strands of DNA. Your answer must include a...
Describe DNA replication on the leading and lagging strands of DNA. Your answer must include a description of the roles of the following enzymes: -DNA polymerase I (5’-3’polymerase activity, 3’-5’ exonuclease activity, 5’-3’ exonuclease activity) -DNA polymerase III (5’-3’polymerase activity, 3’-5’ exonuclease activity) -helicase, primase, ligase, gyrase
Which aspect of the E. coli DNA replication machinery accounts for high processivity? a. The beta...
Which aspect of the E. coli DNA replication machinery accounts for high processivity? a. The beta sliding clamp of DNA Polymerase III b. 3’-5’ exonuclease activity of DNA Polymerase I c. 5’-3’ exonuclease activity of DNA Polymerase I d. Single strand DNA binding protein (SSB) e. The core subunit of DNA Polymerase III An explanation of the solution would be greatly appreciated, thanks!
What would be the effect of each type of the following mutations? a,) Mutation that destroys...
What would be the effect of each type of the following mutations? a,) Mutation that destroys the function of eukaryotic DNA polymerase alpha. b.) A mutation that destroys the 5' to 3' exonuclease activity of DNA polymerase I.
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of...
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3' exonuclease activity of DNA polymerase High fidelity of DNA replication is ensured by 3-5' exonuclease activity of DNA polymerases Next
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of...
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3 exonuclease activity of DNA polymerase High fidelity of DNA replication is ensured by 3-5' exonuclease activity of DNA polymerases Next
4. The enzyme DNA polymerase has two important activities: 5-10-3 polymerase and 3 '-10-5'exonuclease. The 5'-to-3'...
4. The enzyme DNA polymerase has two important activities: 5-10-3 polymerase and 3 '-10-5'exonuclease. The 5'-to-3' polymerase makes one misincorporation per 100,000 incorporations. A misincorporation occurs when the "wrong" nucleotide-residue is added to the growing DNA-molecule, such that the added residue is NOT complementary in the normal Watson-Crick way (G with C, A with T) to the residue in the template DNA. Normally, the 3'-to-5'exonuclease corrects most of these misincorporations, reducing the misincorporation rate to one in 10,000,000. If a...
Question 5 4 pts Which of the following statements in NOT true about DNA replication? DNA...
Question 5 4 pts Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3 exonuclease activity of DNA polymerase ! High fidelity of DNA replication is ensured by 3-5 exonuclease activity of DNA polymerases Next
· If there was a mutation that caused the DNA polymerase I enzyme to fold in...
· If there was a mutation that caused the DNA polymerase I enzyme to fold in a 3D configuration that rendered it non-function, how would the leading strand of DNA synthesis be affected?
Nick translation is an example of the process that would occur when DNA polymerase I is...
Nick translation is an example of the process that would occur when DNA polymerase I is replacing an RNA primer with DNA. Which of these activities would be involved in nick translation? 3'-5' exonuclease and 5'-3' polymerase 5'-3' exonuclease and 5'-3' polymerase 5'-3' exonuclease and 3'-5' polymerase 3'-5' exonuclease and 3'-5' polymerase
Compare the activities of E. coli DNA polymerase III and RNA polymerase by describing the following...
Compare the activities of E. coli DNA polymerase III and RNA polymerase by describing the following properties of each: DNAP III (Replication) RNAP (Transcription) Substrate(s) i.e. what does the enzyme link together Name for the site of enzyme binding Is a primer required? What serves as template(s)? End product Direction of polymerization
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3' exonuclease activity of DNA polymerase High fidelity of DNA replication is ensured by 3-5' exonuclease activity of DNA polymerases Next
Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3 exonuclease activity of DNA polymerase High fidelity of DNA replication is ensured by 3-5' exonuclease activity of DNA polymerases Next
4. The enzyme DNA polymerase has two important activities: 5-10-3 polymerase and 3 '-10-5'exonuclease. The 5'-to-3' polymerase makes one misincorporation per 100,000 incorporations. A misincorporation occurs when the "wrong" nucleotide-residue is added to the growing DNA-molecule, such that the added residue is NOT complementary in the normal Watson-Crick way (G with C, A with T) to the residue in the template DNA. Normally, the 3'-to-5'exonuclease corrects most of these misincorporations, reducing the misincorporation rate to one in 10,000,000. If a...
Question 5 4 pts Which of the following statements in NOT true about DNA replication? DNA replication involves synthesis of multiple RNA primers DNA ligase is needed for successful DNA replication DNA replication requires deoxyribonucleotide triphosphates High fidelity of DNA replication is ensured by 5-3 exonuclease activity of DNA polymerase ! High fidelity of DNA replication is ensured by 3-5 exonuclease activity of DNA polymerases Next
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