· If there was a mutation that caused the DNA polymerase I enzyme to fold in a 3D configuration that rendered it non-function, how would the leading strand of DNA synthesis be affected?
DNA polymerase I plays a major role in proof reading mechanism and polymerase activity. It has both 5' to 3' and 3' to 5' exonuclease activity. 3' to 5' exonuclease activity used in proof reading or in DNA repair mechanism if any mismatch occurs during the DNA replication.
Due to mutation in DNA polymerase I it not do proof reading activity or DNA repair mechanism so lot of mutation occurs in Leading strand.
· If there was a mutation that caused the DNA polymerase I enzyme to fold in...
What would be the effects on DNA replication if mutation of DNA polymerase III caused it to lose each of the following activities: 1. 5' to 3' polymerase activity 2. 3' to 5' exonuclease activity:
20. Which enzyme separates the strands of the DNA helix? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 21. Which enzyme joins newly synthesized DNA fragments on the lagging strand? A. DNA Polymerase E. Single Stranded Binding Proteins B. Ligase F. Primase C. Helicase G. Lagging Strand D. Topoisomerase H. Leading Strand 22. In a PCR reaction, at which temperature do the two strands of DNA...
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30. DNA polymerase cannot begin the synthesis of a daughter strand and depends on an enzyme called primase to build a short segment of RNA called an RNA _ at the start of the leading strand and at the start of each Okazaki fragment of the lagging strand. Label both of these short RNA segments
both stranas es:COVID mation & es Question 2 DNA polymerase III (DNA pol III) is needed for leading strand synthesis o lagging strand synthesis both strands D Question 3 ONE RNA primer is needed for o both strands O lagging strand synthesis leading strand synthesis Question 4 Multiple RNA primers are needed for O both strands leading strand synthesis lagging strand synthesis Question 5
Normally, DNA polymerase III proofreads as it synthesizes a new strand. If it misses an error and adds an incorrect base to a new strand, what is the consequence? This is a mutation and would be repaired by ligase because cells cannot have mutations that are uncorrected. This is a mutation and would result in the death of the cell that contains it. This is a mutation and would be inherited by cells that receive copies made from the altered...
Match the following (Total 24 pts) Enzyme responsible for joining DNA strands together The nascent DNA strand that is being synthesized in the same direction as the replication fork. Enz 39 DNA polymerase 40 DNA helicase responsible for transcribing RNA 41DNA sliding clamp 42 Single Stranded Binding Proteins DShort, newly synthesized DNA fragments 43 RNA primer 44 DNA ligase formed on the lagging template strand E Abundle or proteins that assist in the rate of transcription of DNA to mRNA....
Suppose a mutation occurs in the gene encoding eukaryotic RNA polymerase I, II, or lll that renders that polymerase non-functional. Match each RNA polymerase mutation with all of the cellular processes that it would disrupt. Mutation in eukaryotic RNA polymerase I Mutation in eukaryotic RNA polymerase II Mutation in eukaryotic RNA polymerase III pre-mRNA processing RNA synthesispre-mRNA synthesis RNAi-mediated gene regulation IRNA synthesis mRNA translation rRNA processing
Question 12 100 pts The fact that DNA synthesis inhibitors in humans prevents retroviruses from being infective led to hypothesize the existance of? DNA polymerase reverse transcriptase restriction enzymes telomerase Question 11 In the synthesis of the prokayotic DNA, this keeps the fork from re-annealing 100 pts Poll multiple RNA primers primane O helicane binding protein Question 13 100 pts Analysis of a cell line that rapidly transforms into a tumor cell line demonstrated an increased mutation rate within the...
Which is not one of the elements needed to amplify DNA by polymerase chain reaction (PCR)? Question 16 options: A) Nucleoside triphosphates that serve as the source of the nucleotides A, T, C, and G needed in the synthesis of the new strands of DNA B) A restriction endonuclease enzyme that cleaves DNA at specific locations C) The segment of DNA that must be copied D) A DNA polymerase enzyme that will catalyze the synthesis of a complementary strand of...
Which of the following is an example of a spontaneous mutation? Yeast DNA is exposed to acridine dye, resulting in distortion of the DNA double helix. Human cells are exposed to X-rays for a prolonged period of time, leading to multiple DNA strand breaks. DNA polymerase III accidently mismatches a G to a T during Escherichia coli DNA replication, resulting in a silent mutation. Plasmodium vivax cells are exposed to ultraviolet radiation, leading to the formation of thymine dimers within...