Question

You identify a missense mutation in a gene called doublecortin (DCX). This missense mutation has been shown to cause licencephaly in humans, a dominant disorder that impairs brain development. Which of the following reverse genetic methods could be used to create a mouse model of this devastating disease using this specific missense mutation as a starting point (circle all that apply)?

a. RNA interference (RNAi).

b. Ectopic expression of a transgene

c. CRISPR

d. An enhancer screen

e. A suppressor Screen

Answer 1

The following reverse genetic methods could be used-

Rna Interferences-This is because we Can obtain partial loss-of-functiion using this technique as it is heritable.

b. Ectopic expression of a transgene-Can easily create a missense mutation