Question

What are the general issues with the  bacterial expression system for making recombinant proteins?

Answer 1

1. difficulty in expressing mammalian proteins

2. accumulation of proteins as inclusion bodies

3. protease contamination from host proteins leading to expressed protein's degradation.

4. It can also result in endotoxin accumulation

5. Eukarytotic and posttranslational modifications are not possible

6. produces insoluble proteins and poor expression.

7. lack of chaperons required for protein folding

8. limited ability to carry out extended disulphide bond formation

9. codon bias occurs whcih hampers protein synthesis and gene expression in bacteria