0.2 mls of a 10-4 dilution of a virus preparation yields 90 plaques. What is the...
0.2 mls of a 10-4 dilution of a virus preparation yields 90 plaques. What is the number of PFU per ml in the undiluted virus preparation
Homework Answers
PFU (plaque forming unit) is the number of viral particles forming plaque per unit volume.
using the formula:
number of plaques formed / (dilution factor 
volume of diluted virus per well)
given: number of plaques formed = 90
number of dilutions = 10^4, this shows the sample is diluted 4 times in 10 ml of dilution per well.
dilution factor will be 1/10^4 = 0.0001
volume of diluted virus added = 0.2 ml
the number of plaques formed per unit in an undiluted sample =
pfu/ml
hence, the undiluted sample contains 4.5 106
pfu /ml.
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