How is it possible that different mutations can result in the loss of a RFLP? How...
How is it possible that different mutations can result in the loss of a RFLP?
How are positive YAC clones selected?
What is the purpose of digesting the YAC with BamH and SnaB1?
Homework Answers
1. Different mutations can either destroy pre-existing sites or create additional restriction sites which can result in the loss of a RFLP.
2. Positive YAC clones obtained from screening the library and identified by PCR or colony hybridization.
3. BamH and SnaB1 cleave YAC circularly as BamH1 cuts at telomeres and SnaB1 in middle of SUP4 selectable marker.
Thanking you
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