Explain how DNA methylation could be used to regulate gene expression in a tissue-specific way. When and where would de novo methylation occur, and when would demethylaiton occur? What would occur in the cells that give rise to eggs and sperm (germ-line cells)
Explain how DNA methylation could be used to regulate gene expression in a tissue-specific way. When...
Explain how DNA methylation could be used to regulate gene expression in a tissue-specific way. When and where would de novo methylation occur, and when would demethylaiton occur? What would occur in the cells that give rise to eggs and sperm (germ-line cells)
In eukaryotic cells, there are specific transcription factors (activators) that regulate gene expression. Describe the organization of eukaryotic genes (i.e. promoters/enhancers) and how transcription factors are able to control gene expression and enzyme activity. a) Throughout the course we discussed the enzyme lactase and how it is differently regulated (and therefore expressed) in different human populations. Describe how this gene is differently expressed in different populations and when this/these change(s) are predicted to have taken place. b) Differential gene expression...
What control elements regulate expression of the mPGES-1 gene? The promoter of a gene includes the DNA immediately upstream of the transcription start site, but expression of the gene can also be affected by control elements. These can be thousands of base pairs upstream of the promoter, grouped in an enhancer. Because the distance and spacing of these control elements make them difficult to identify, scientists begin by deleting sections of DNA that contain possible control elements and measuring the...
"In one model/diagram, show how transciption factors, histone modifiers, chromatin remodelers, DNA methylation all cooperate to regulate the thermostat for an individual gene (how much mRNA is transcribed)." Lets say you've discovered a gene tht codes for a protein that interferes with your ability to scratch-off quiz cards. Clearly you need to shut this gene off. You can name your gene and its regulatory transciption factor(s) whatever you want, but the rest of your model should be accurate. Make sure...
You are conducting an experiment identifying enhancers that
regulate the expression of a gene that codifies for a protein that
participates in cell division. The gene is called Mitosis
Regulatory Protein A or MRPA. The complete DNA sequences
for the MRPA promoter and coding region have been
identified. However, it is unknown if MRPA has enhancers
regulating its transcription. To answer this question your lab
first produced a transgenic cell line where GFP has been inserted
as a reporter gene....
Two identical twins who showed very similar methylation patterns when they were 4 years old show very divergent patterns at age 40. This divergence is most likely due to a different alternative splicing in the two individuals Ob different levels of histone acetyltransferase in the two individuals. Oc environmental differences that they experienced as adults. O d. different genomic imprinting in the two individuals. O e differences in their experiences in utero Suppose that a certain enzyme is synthesized whenever...
C . Give the specific that explain how hormones controls control gene expression in e O 32. Define Epigenetic Oby. 33. This is a hypothetical model of interrelated gene circuits is given below. RO operator and SG- structural pene. The Repressor may be a inactiverective regressor and the effector may be an of interrelated gene circuits is given below. RG-regulator gene, OG er or corepressor B ressors B & C at the time of State the effect on structural genes...
27. RNA Polymerase ence of DNA that controls gene expression G. binds an operator and stops gene expression in LAC operon by preventing RNA polymerase from binding gene and transcribing. H. Duplication of DNA in Sphase of Interphase 28. Codon 29. Transgenic 30. Recombinant DNA 31. PCR 32. 33 34. 35. 36. 37. 38. Plasmid Gene Therapy Gel electrophoresis DNA Profile DNA ligase GMO concern GMO benefit A. Analyzing STR patterns of blood or hair samples to solve crimes B....
Label the following as either proto-oncogene, oncogene or tumor suppressor 3. A gene that initiates apoptosis when DNA damage occurs a. b. A gene that initiates the transition from G1 to S phase A gene that inhibits the transition from G1 to S phase c. d. A gene that promotes cell growth, combined with a hyperactive promoter How would methylation of the promoter of the gene in question 3c affect the cell? Be specific using what you know about the...
Explain the results in the Methylation Specific PCR (MSP)
above. Be specific
The positive control in the lower panel is much stronger than
the patient samples. What control would be better (or in addition
to this control) to detect the weak expression in T3 and T4? Be
specific
Clinical Background: A 40-year-old male patient complained of a right frontal headache and right neck pain for the duration of 4 weeks. The pain was associated with intermittent blurred vision in the...