why does DNA when denatured to ssDNA at high temperatures not renature completely to its original form dsDNA when cooling?
why does DNA when denatured to ssDNA at high temperatures not renature completely to its original...
Single stranded DNA (ssDNA) is a product of DNA denaturation. When compared to double stranded DNA (dsDNA) it is much more flexible with its persistence length (lp) estimated to be an order of magnitude lower than that for dsDNA. If a particular dsDNA sequence has a (4-63 nm): a) Estimate at what concentration of NaCl dsDNA flexibility is comparable to that of ssDNA [NaCl]- a) Estimate at what concentration of MgCl2 dsDNA flexibility is comparable to that of SSDNA [MgCl2]-
recombinant DNA experiment Select the correct words from the drop-down menus for all 4 options ( 1 mark ) Q1) After adding the cell lysis buffer and incubating, neutralisation enables the separation of plasmid DNA from chromosomal DNA based on their different sizes. On neutralisation, the single–stranded DNA species of both chromosomal and plasmid origin (previously separated or denatured by alkali treatment) tend to re-anneal or ‘renature’, but… (i) The Choose. (plasmid / bacterial chromosomal ) DNA can re-anneal faithfully...
Why does tungsten undergo a magnetic transition from paramagnetism to diamagnetism at high temperatures?
1. Explain why the salicylic acid is insoluble at RT and soluble at high temperatures (when hot)? -Explanation should include what is occurring/ happening in molecular level. -Your answer should be concise and should not exceed 100 words (please note that , if it is too long it will not be graded)
Why does high stringency ensure that a positive result is the result of the probe binding to its correct target DNA rather than to non-target sequences? (Hint: How does temperature and salt concentration affect the properties of double-stranded DNA and why might this be important for hybridization?)
Why does snow remain on mountain slopes even when daytime temperatures are higher than the freezing temperature?
Explain in detail: Why does DNA form a double-helical structure?
question 3: what happens to the structure of a protein when it is
denatured?
question 4: why is it important for a protein to be at a
certain ph to carry out its function
zymes in your textbook, especially the sections on enzym REPORT: Your report should include two separate files: a table of the data from your enzyme experiments online and conclusions consisting of answers to the questions. WARM-UP QUESTIONS: 1. What part of the name 6-O-a-L-ramnosyl-D-glucosidase shows that...
Why does the original color of the object turn into orange when temperature increases above room temperature?
does corrosion increase with high ph or low and why? what is its effect in tubes