Calculate the Rf using the following equation:
Rf = distance pigment migrated (mm) / distance solvent migrated (mm)
|
Table 1: Part 1: Chromatography Data (15 points) |
|||
|
Solvent |
Distance from Original Line to Solvent Front |
Number of Bands |
Rf Factor |
|
Acetic Acid |
9cm |
1 |
? |
|
Acetone |
7.2cm |
3 |
? |
|
Mineral Oil |
5cm |
1 |
? |
|
Water |
8.8 |
0 |
? |
You have to taken silvent which is moved to the farthest distance in the chromatography. In this case solvent is acetic acid.
Now you have to divide each solvent cover distance to solvent distance on chromatography.
| Solvent | Rf value |
| Acetic acid | 9/9=1 |
| Acetone | 7.2/9= 0.8 |
| Mineral oil | 5/9= 0.55 |
| Water | 8.8/9= 0.91 |
Calculate the Rf using the following equation: Rf = distance pigment migrated (mm) / distance solvent...
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Distance traveled by the solvent:
cm
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