Question

1. An oligopeptide X was obtained from blood plasma with the sequence W-P-T-I-H-E.

(a) Draw the structure of this X oligopeptide in a form that would predominate at pH 7.0

(b) Calculate the overall net charge of X oligopeptide at that pH (7.0) to the nearest 0.01

(with a precision of two decimal places)?

(c) In running through an ion-exchange column made up of sulfate S-resin, what pH fraction would this X oligopeptide be expected in a gradient of acid-to-base elution?

Answer 1