Question

you have developed a genetically engineered bacterial strain that can make extremely large amounts of human catalase, but so much so that the catalase becomes an insoluble aggregate and has no enzymatic activity. which of the following procedures would NOT help you obtain soluble, enzymatically active catalase?

A. Make the bacteria over produce the chaperone proteins in addition to the catalase.

B. Try dissolving the catalase in urea, then slowly remove the urea until enzymatic activity returns.

C. Use a protease to cleave into smaller , more soluble fragments.

D. All of these procedures would work.(none)

Answer 1

Option C is incorrect as catalase are very stable enzyme and are very resistant to proteolysis. Even if proteases cleave an enzyme it will lost its primary structure and lost its activity forever.