Question

You wish to produce a human enzyme, protein A, by introducing its gene into bacteria. The genetically engineered bacteria make large amounts of protein A, but it is in the form of an insoluble aggregate with no enzymatic activity. Which of the following procedures might help you to obtain soluble, enzymatically active protein? Select all options that may be useful.

Dissolve the protein aggregate in urea, then dilute the solution and gradually remove the urea.

Treat the insoluble aggregate with nuclease.

Make the bacteria synthesize protein A in smaller amounts.

Treat the insoluble aggregate with a protease.

Make the bacteria overproduce chaperone proteins in addition to protein A.

Answer 1

A dissolve the protein aggergate in urea, protein will completely soljblized in urea an on slow dilution will allow the proteins to refold and it will retold into its proper conformation

So answer will be the first one