What key reaction differentiates Escherichia coli from Klebsiella and Enterobacter? What key reaction differentiates Klebsiella from...
QUESTIONS 1) What do Escherichia coli, Enterobacter cloacae and Klebsiella pneumoniae have in common? Are the IMVIC tests alone enough to differentiate these bacteria? TU 3WARAIA COCnao 2) What is the purpose of the IMViC tests? Explain. YdLE 3) List the four tests included in the IMVIC series. What is the purpose of each of the tests? What are the important ingredients of each? - 150
what results would you get when testing these organisms (Klebsiella pneumoniae, Escherichia coli, Staphylococcus saprophyticus, Proteus mirabilis, Proteus vulgaris, Providencia stuarti, Pseudomonas aeruginosa, Enterobacter aerogenes, Morganella morganii) Tests being run: MacConkey agar test, a Gram staining test, SIM agar and Urea agar (just indicate if results are negative or positive)
6. Refer to Tables 13.2 and 13.3 and classify Escherichia coli, Bacillus subtilis and Enterobacter aerogenes as Gram-positive, Gram-negative, coliform or non-coliform a. E. coli- b. B. subtilis - C. E. aerogenes- 7. What is the role of eosin and methylene blue in EMB agar? 8. Why do fermenters produce dark colonies on EMB agar? 4 Page
Suggest a way to distinguish Citrobacter freundii from Escherichia coli and from Proteus species.
10. IMViC stands for what tests? What results are expected for E. coli and Enterobacter aerogenes? What reagent(s)-indicator(s) give the reactions? How do positive tests look?
1. The Gram reaction; Gram Positive or Gram Negative 2. The shape of the cells; bacillus, coccus, spiral 3. The typical arrangement of the cells; single, pairs, clusters, chains etc. Shape of cells Arrangement of cells Name of organism Gram Reaction Bacillus cereus Enterobacter aerogenes Enterococcus durans Escherichia coli Micrococcus luteus Senatra marcescens Staphylococcus epidermidis streptococcus Salivarius Klebsiella pmeumonia Salmonella entertidis Shigella Sonnei Staphylococcus aureus Streptococcus pyogenes *Hint: The organisms used in the Lab. are listed in the Safety Guidelines...
Build case study for Escherichia coli. What would be the patient history and symptoms, how to collect specimen from patient to test, how lab will identify/confirm pathogen (diagnosis), treatment, prognosis, and virulence factors.
Pseudomonas stutzeri derives more energy from NO_3 reduction than Escherichia coli. Explain one reason for this difference.
One of the Escherichia coli mutants is always producing β-galactosidase. What is the term for this type of expression? In this experiment we have a wild type lac gene, mutated lacI gene, and a mutated lacZ gene. Thank you!
10. Fill in the blanks in this diagram to make a key for these 3 bacteria: Escherichia coli, Enterobacter aerogenes and Staphylococcus aurvens. Growth on EMB No es Metallic green colonies Thick mucoid pink colonies Growth on Mannitol Salt Agar Genus: Gram stain: Genus: Gram stain: Yes with yellow zone around growth Genus: Gram stain: 11. How can one distinguish the 3 common urinary tract pathogens on the HardyCHROMTM UTI demo plate? 12. What is the value of making a...