Question

An experiment, in the Cellular and Molecular Biology lab,on the Chlamydomonas reinharditii was done. The effects of the Neomycin and Cyclohexamide were observed. Their effect on the flagella and their regeneration was observed. So, under the microscope, the number of NONMOVING cell were counted. Please write the hypothesis, null hypothesis, prediction, and explanation for each Neomycin and Cyclohexamide (separately). Please write if the number of NONMOVING cells will increase or decrease when neomycin is applied and when cyclohexamide is applied.

Thank you!

Answer 1

when both the flagella are amputated, on application of cyclohexamide which will inhibit formation of proteins, is known to cause regrowth of less than one - third of each flagellum. on the other hand if only one of the flagella are removed and the effects are then observed, then the regrowth levels of removed flagellum are found to be greater as compared to when both were removed. therefore in first case, where both flagella were removed, nonmoving cells would increase in number.

on application of neomycin, before exposure of chlamydomonas to stress environments or removal of flagella, it is observed that deflagellation was prevented whereas if neomycin was added after flagella removal, did not block flagellar assembly which can result in decrease in number of nonmoving cells under microscope.