0.1 ml of a 10-4 dilution of M. luteus was plated. After incubation, 80 colonies were...
0.1 ml of a 10-4 dilution of M. luteus was plated. After incubation, 80 colonies were counted. How many colony-forming units per ml were in the original E. coli sample?
Homework Answers
CFU per mL = number of colonies / (volume plated × dilution)
Colonies = 80
Dilution = 10^-4
Volume plated = 0.1 mL
CFU per mL = 80/(10^-4 × 0.1) = 80 × 10^5 = 8 × 10^6 CFU per mL
Answer is 8 × 10^6 CFU per mL
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advance.
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please help with whatever possible. thank you so much in
advance.
Name One use of serial dilutions is to calculate the concentration of microorganisms. Since it would usually be challenging or even impossible to actually count the number of microorganisms in a sample, the sample is diluted and plated to get a reasonable number of colonies to count (usually between 25 to 250 colonies is the goal). Since each colony on an agar plate theoretically grew from a single microorganism,...
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help me with the math.
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