Enzyme kinetics: You recently joined a new research group, and in your first week you successfully...

Question

Question

Enzyme kinetics: You recently joined a new research group, and in your first week you successfully...

Enzyme kinetics: You recently joined a new research group, and in your first week you successfully discover a new version of an enzyme called happyase, which you name happyase*, that catalyzes the chemical reaction:

HAPPY ↔ SAD

You begin devising ways to characterize the enzyme. (5 points total, 2 each for A, B and 1 for C).

In the first experiment, with [E_t] at 4 nM, you find that the V_max is 1.6 mM/s. Based on this experiment, what is the k_cat for happyase*? (Include appropriate units.) (2)

In another experiment, with [E_t] at 1 nM and [HAPPY] at 30 mM, you discover that V₀ is equal to 300 nM s^-1. What is the measured K_m of happyase* for its substrate HAPPY? (Include appropriate units.) (2)

science biology

Add a comment Improve this question Transcribed image text

Answer 1

Answer #1

Add a comment

Answer 2

Similar Homework Help Questions

Enzyme kinetics: You recently joined a new research group, and in your first week you successfully...

Enzyme kinetics: You recently joined a new research group, and in your first week you successfully discover a new version of an enzyme called happyase, which you name happyase*, that catalyzes the chemical reaction: HAPPY ↔ SAD You begin devising ways to characterize the enzyme. (5 points total, 2 each for A, B and 1 for C). In the first experiment, with [Et] at 4 nM, you find that the Vmax is 1.6 mM/s. Based on this experiment, what is...
QUESTION 3 The enzyme happyase catalyzes the following reaction: SAD = HAPPY An enzyme kinetics experiment...

QUESTION 3 The enzyme happyase catalyzes the following reaction: SAD = HAPPY An enzyme kinetics experiment gave the data shown in the table below. The concentration of happyase used in the experiment was 25.00 UM. [SAD] (MM) 1.000 2.000 3.000 4.000 Vo (mm/s) 3.700 6.727 9.250 11.385 Determine the value of Vmax for happyase (in mM/s). QUESTION 4 Using the information above, determine the value of Km for happyase (in mm). QUESTION 5 How long (in milliseconds) does a single...
Question 13 of 49 A research group discovers a new version of happyase, which they call...

Question 13 of 49 A research group discovers a new version of happyase, which they call happyase,that catalyzes the chemical reaction Map HAPPYSAD The researchers begin to characterize the enzyme a In the first experiment, with [El at 6 nM, they find that the Vmax is 3.50 Ms Based on this experiment, what is the keat for happyase ? In another experiment, with [E] at 3 nM and [HAPPY] at 40 HM, the researchers find that Vo 1390 nM s1....

Question 1 (2 points, 1 point for each correct answer) A research group discovered a new...

Question 1 (2 points, 1 point for each correct answer) A research group discovered a new enzyme happyase that catalyzes chemical reaction SAD STUDENT HAPPY STUDENT Researchers characterized this enzyme and found that at [E,-10 nM this enzyme has Vmax at 2.5 μM/s. It was also experimentally measured that at [SAD STUDENT)-0.05 mM this enzyme has Vo -500 nM/s. What are the kat and Km of the happyase enzyme? Make sure to write down units of measure! Answers: keat- Question...
. ous enzyme 'happyase catalyzes t the conversion of SAD to HAPPY with a Keat 100...

. ous enzyme 'happyase catalyzes t the conversion of SAD to HAPPY with a Keat 100 sec 40 35 30 25 20 10 10 20 30 40 50 60 70 90 100 110 ISADI QIM A kinetic experiment was conducted and the intitial velocity of the reaction as a function of SAD concentration is shown above. The initial velocity at 1 mM [SAD] was measured to be 40 nM/sec. a) What is the total [happyase] used above (make sure to...
You are still interested in the enzyme happyase, which catalyzes the following reaction: HAPPY = SAD...

You are still interested in the enzyme happyase, which catalyzes the following reaction: HAPPY = SAD Previously, you determined that kcat = 400 s and Km = 10 M for your sample of happyase. Further research shows that this happyase sample was actually contaminated with a reversible inhibitor called ANGER. When ANGER is fully removed from the happyase preparation and [Eltotalis 4 nM, the measured Vmax is increased to 4.8 umes-1 and the measured Km is now 15 MM. Use...

Question 2: You have just discovered a new enzyme that catalyzes the degradation of cellulose to glucose. You hope to commercialize this enzyme ultimately to invent a process for biofuel manufact...

Question 2: You have just discovered a new enzyme that catalyzes the degradation of cellulose to glucose. You hope to commercialize this enzyme ultimately to invent a process for biofuel manufacture. In order to determine how efficient the enzyme is and how fast the enzyme catalyzes the reaction, you have to perform some basic enzyme kinetics. Plot the following enzyme data for the enzyme catalyzed reaction and determine the Km and Vmax. Show your graph with correct units on the...
For her undergraduate project, Jessica studied an enzyme that catalyzes the reaction A↽−−⇀B. For substrate A,...

For her undergraduate project, Jessica studied an enzyme that catalyzes the reaction A↽−−⇀B. For substrate A, she determined that ?m=2.5 μM and ?cat=35 min−1. Jessica graduated and her project has been passed on to you. Unfortunately, Jessica was so busy that she sometimes forgot to record all of the details of an assay in her lab notebook. Your mentor suggests that you try to back calculate some of the missing concentration values. Assume that the enzyme follows Michaelis–Menten kinetics. 1)...
Interpret the data above 1. What is the aporoximate Km and Vmax (units matter) 2.Briefly explain...

Interpret the data above 1. What is the aporoximate Km and Vmax (units matter) 2.Briefly explain how you found each 3.if you used 0.020 microM enzyme in your studies, what is kcat in units of s^-1? Show your work units matter 4. Does this enzyme appear to display cooperativity? Explain how you came up to that conclusion. Directions: Below are data from 4 separate experiments that you must analyze/evaluate. Using the information from all 4 experiments, you must then propose...

Enzyme kinetics: You recently joined a new research group, and in your first week you successfully...

Homework Answers