What reaction do DNA polymerases catalyze. What are the specific substrates they use? What are their limitations?
What reaction do DNA polymerases catalyze. What are the specific substrates they use? What are their...
53. RNA polymerases differ from DNA polymerases in that A. RNA polymerases are less effective at proofreading than DNA polymerases. B. only DNA polymerases are processive. C. RNA polymerases do not require a template. B. DNA polymerases use ribonucleoside triphospates as substrates. E. RNA polymerases use deoxyribonucleoside triphosphates as substrates. 54. Which of the following might be a tRNA anticodon for leucine? A. 5-AAC-3' B. 3'-AAC-5'. C. 5'-TTC-3'. D. 3'-UUG-5'. E. 5-UUG-3'. 55. During protein synthesis, ribosomes A. translate mRNA...
How do enzymes catalyze reactions? A) Enzymes bind with and change the conformation of substrates. B) Enzymes change the free energy of the reactants. C) Enzymes increase the activation energy of a reaction. D) Enzymes carry out a reaction without needing reactants. E) Enzymes are denatured by a reaction to catalyze it.
. What enzymatic activity displayed by DNA Pol I do eukaryotic polymerases lack that is compensated for by the activities of Fen1 and RNase H?
Compared to RNA elongation by RNA polymerases, DNA elongation by DNA polymerase requires an additional reaction component. Which is appropriate for fulfillment of this requirement? Select one: a. a primase is needed for the biological DNA replication. b. a short DNA primer is necessary for PCR. c. a host tRNA is used for the synthesis of DNA by viral reverse transcriptase. d. All of these e. None of these
a. What reaction do lipase catalyze? c. Which drug targets a lipase? How is it specific for that one lipase? 2. For the following answer - brain, liver, muscle, intestines, RBC, or adipose (there can be more than one answer or none). a. Synthesize(s) triacylglycerols? b. Synthesize(s) VLDL? c. Synthesize(s) chylomicrons? d. Synthesize(s) fatty acids for energy storage? e. Store(s) triacylglycerols? f. Can undergo beta-oxidation for energy?
Thermostable DNA polymerases are used in PCR because they a. bind DNA more efficiency than thermolabile DNA polymerases b. do not have a proof-reading activity c. are less expensive than E. Coli DNA Pol III d. can survive at the high temperatures used to denature DNA e. are water soluble
What other DNA polymerases are involved in DNA replication and when are they active? Although Dpb3 and Dpb4 are considered non-essential, DNA polymerase ? dissociates more frequently from the template when Dpb3 and Dpb4 are deleted. What are the explanations for this result? What do you think would happen if, in addition to Dpb3 and Dpb4 deletion, the exonuclease activity of Pol2 was also mutated in such a way that it did not function? How might replication fork progression and...
During DNA replication in humans (eukaryotes), what polymerases (1,2,3?) are involved?
I-Upon heavy damage to the cell'S DNA, the normal y stall when encountering replicative DNA polymerases ma damaged DNA, triggering the use of backup translesion polymerases. These backup polymerases (2 marks) A. have 5'-to-3' exonucleolytic proofreading activity B. are substituted by the replicative polymerases after adding only a few nucleotides. C. can generate mutations only on undamaged DNA D. may identify specific D NA damage and add the suitable nucleotide to restore the original sequence. E. All of the above...
Lesion bypass DNA polymerases that can “read through” things like thymine dimers are much more error-prone than other DNA polymerases? Why do you think this is? What is the benefit of having them if they are mutagenic?