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list in steps along with a picture the mechanism of penicillin acylase

list in steps along with a picture the mechanism of penicillin acylase
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Answer #1

6 - APA is a beta lactam compound.

Oxidation of phenyl acetic acid precursor to o - hydroxy phenyl acetic acid.

The penicillin is converted into 6 - APA by the reaction of removing the side chain linked to amino group in position 6. It is achieved through enzymatic processes using enzymes like penicillin acylase. The function of this enzyme is to recognise a side chain and break the peptide linkage joining it to the 6 - APA nucleus.

The reaction takes place in aqueous solution with alkaline pH j6 APK is extracted by adjusting the pH to the isoelectric point This is followed by additional aqueous phase extraction so it may be recycled as a precursor in the fermentation.

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