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- Given there are 20 possible amino acids and a stop codon, what is the theoretical...

- Given there are 20 possible amino acids and a stop codon, what is the theoretical maximum size of a CII protein library generated by a primer containing i) 9 degenerate positions, ii) 3 degenerate positions.

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Answer #1

A. 9 degenerate positions would mean the code should be made of 9 nucleotides . Hence the permutation combination would be which is equal to 262144 hence it can be concluded that 262143 codons code for amino acid and one codon does not code for any amino acid functioning as a stop codon

B. Similarly 3 degenerate positions would mean code made of 3 nucleotides . The permutation combination would be resulting in 64 codons . In which 63 would code for amino acids and one would act as stop codon

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