What is the importance of growing bacteria into SOB medium before performing inoue’s chemical competence on...
What is the importance of growing bacteria into SOB medium before performing inoue’s chemical competence on it?
Homework Answers
Answer:-
SOB i.e Super Optimal Broth. It is basically a media which nutritionally enriched for the growth of the bacteria. Growing bacteria into SOB medium before performing inoues's chemical competence on it. The reason being is that the cell initially made competent during the transformation of cell where the small hole is made for the foreign genetic material and for all this overall mechanism cell needs the isotonic medium which provides all the essential requirements to the cells as it helps in the preparation of competent cell before transformation.
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