Question

write in your own words the steps to properly using a compound light microscope.

Answer 1

The steps to use a compound microscope step by step:-

1. The first step for using the compound microscope requires turning on the illuminator. It is important to increase the intensity of light slows.
2. The specimen that you want to observe needs to be put on the stage right above the aperture. In order to hold it in right position make sure to hold and fasten it with help of stage clips.
3. Place a cover slip so that the image quality is of high value and quality.
4. The iris diaphragm needs to be completely open and hence this helps in allowing more light to enter. This helps in controlling the light entered and the resolution.
5. After this rotate the nosepiece in a way that objective lens is placed directly above the sample and after that the iris diaphragm needs to be adjusted. This helps in penetration of proper light.
6. In order to focus on the specimen, it is important to adjust the coarse knob and hence adjust the focus. After this in order to bring high quality and sharp focus, turn and adjust the fine adjustment knob
7. The viewer must be able to rotate and adjust the nosepiece settings and hence the focus can be done