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Promoters were originally identified as consensus sequences upstream from transcriptional start sites. Part A What additional...

Promoters were originally identified as consensus sequences upstream from transcriptional start sites.

Part A

What additional evidence might support the assignment of these sequences as parts of promoters?

Check all that apply.

Methods of identification of transcription start sites that look like the consensus sequences.
There are no known endonuclease that is capable to cleave these highly conservative sequences.
These sequences are the only recognized by σ subunit of corresponding RNA polymerase.
Mutations engineered in the site under study should alter the rate of transcription of adjacent genes.
RNA polymerase binds tightly to DNA in the vicinity of these consensus sequences.
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