If there were a mutation to aldolase in which the active site aspartate was mutated to...
If there were a mutation to aldolase in which the active site aspartate was mutated to tyrosine, how might the mutation affect the kinetics of the aldose? Would the enzyme be catalytically competent? Which kinetic parameters would you expect to be affected and how?
Homework Answers
Aldolase is an enzyme which catalyzes the conversion of fructose-1,6-bisphosphate into triose phosphates. The active site of the aldolase contains three amino which determines the activity and catalytic power of the enzyme, aspartate, lysine and serine. The mutation in the aspartate reduces the activity of the enzyme. The mutated enzyme has only 1.5 percent of the activity of the wild-type enzyme.
There is no change in the binding site of the substrate. The mutated enzyme is still able to catalyze the reaction, and is catalytically competent with reduced catalytically activity.
The kinetic parameters which are affected by the mutation:
The rate of reaction(Vmax) is decreased drastically. Rate constant and substrate reaction remains unchanged.
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If there were a mutation to aldolase in which the active site
aspartate was mutated to...
13 14 Search 13 How will a mutation in the active site df an enzyme that...
13 14
Search 13 How will a mutation in the active site df an enzyme that reducing substrate binding affect enzyme parameters? OS (2 points) Reduce Vm Reduce Km Increase km Reduce Vm and km 14 Which amino acid residue sequence would mostly likely compose a transmembrane segment of a protein? (2 Points) VLVAVLESFLLLSL GRRHLGFEATFFQ GRKKRRORRRPQ NVFKGNTISOKIS 15 What would be observed if purified IgG was boiled and not reduced and then applied to SDS- PAGE (2 points)
A substitution mutation occurs which changes the amino acid sequence of the active site in an...
A substitution mutation occurs which changes the amino acid sequence of the active site in an enzyme. What will happen to substrate binding as a result of this mutation? A) It will increase B) It will decrease C) It will be eliminated D) It will be unchanged E) Either B or C F) It depends upon what amino acid is substituted My first choice was E, which is incorrect, my second choice is now F.
several cells were mutated to understand the functions of some organelles. choose which mutation would affect...
several cells were mutated to understand the functions of some organelles. choose which mutation would affect the cell more dramatically and explain why: 1) cell lacking cilia 2) cell lacking ribosomes 3) cell lacking nucleus 4) cell lacking centrioles or 5) cell lacking golgi apparatus
Site-directed mutagenesis techniques were used to synthesize a mutant citrate synthase enzyme in which the active...
Site-directed mutagenesis techniques were used to synthesize a mutant citrate synthase enzyme in which the active site histidine was converted to an alanine. Why did the mutant citrate synthase enzyme exhibit decreased catalytic activity?
Some bacteria make an enzyme called β-galactosidase that catalyzes the breakdown of the polysaccharide β-galactoside into...
Some bacteria make an enzyme called β-galactosidase that catalyzes the breakdown of the polysaccharide β-galactoside into monomers of galactose. A population of bacteria were found with an amino acid mutation within the active site of β-galactosidase protein, and these bacteria were unable to breakdown β-galactoside polymers. A) How would you identify a population of bacteria with mutant β.galac tosidase in terms of the relative concentration of β-galactoside and galactose in their cells? B.) Explain how the amino acid mutation could...
24. Explain the energetic benefits of making the enzyme active state rather than to the substrate...
24. Explain the energetic benefits of making the enzyme active state rather than to the substrate conformation. Also explain how contribute to enzyme action. In other words, how do these weak intera enzyme and the transition state lower the activation energy?) of making the enzyme active site complementary to the transition nation. Also explain how multiple weak interactions words, how do these weak interactions between the 25. 1/F. The pk's of titratable groups of amino acids remain constant irrespective of...
. Gleevec (Imatinib) inhibits protein kinase BCR-ABL, which is constitutively active in patients with Chronic Myelogenous...
. Gleevec (Imatinib) inhibits protein kinase BCR-ABL, which is
constitutively active in patients with Chronic Myelogenous Leukemia
(CML). The structure of Gleevec is shown below:
a) Based on our discussion in lecture and the structure above,
explain how the “lead” compound structure for Gleevec was designed
and how it led to the development a compound that could bind ABL
with higher affinity than ATP.
b) Using a broad kinase inhibition assay, you discover that
Gleevec inhibits another tyrosine kinase called...
Only answer if you have a background in biochemistry. Thanks odon number Normal 506 507 508...
Only answer if you have a background in biochemistry.
Thanks
odon number Normal 506 507 508 509 510 511 Mutant ene (coding strand) ATC ATCTT GGT GTTT gene (coding strand) ATc ATX XXT GGT TCC GTT TCC What effect will this mutation have on the amino acid sequence of the protein encoded by the CFTR A Deletion of a phenylalanine residue with no change in the C-terminus sequence. B. Deletion of a leucine residue with no change in the sequence....
Question 5 Your friend is an amateur mushroom hunter. He accidentally eats an Amanita phalloides "Death...
Question 5 Your friend is an amateur mushroom hunter. He accidentally eats an Amanita phalloides "Death Cap" mushroom and becomes very ill. You recall from university that this mushroom is toxic because it contains a aminitin, a noncompetitive inhibitor of RNA polymerase. Which of the following hypothetical treatments (if they were possible) would NOT help your friend? Select all best answers for full credit Injecting more RNA polymerase into his cells. Injecting a drug that binds the enzyme-binding region a...
Please, need help with this problem ! ( Cell Biology ) Thank You Problem 9 -...
Please, need help with this problem ! ( Cell Biology )
Thank You
Problem 9 - Panoptes In Ancient Greek mythology, the giant Argus was attributed the nickname "Panoptes" ("all seeing") because he had 100 eyes spread all over his head and body. And our friend Argus Panoptes, herem is afflicted with ubiquitous expression of the eyeless gene Aristotle and molecular biology Nowadays, researchers induce mutations in animal models to better understand mutations in humans In fruit flies, a genetic...
13 14
Search 13 How will a mutation in the active site df an enzyme that reducing substrate binding affect enzyme parameters? OS (2 points) Reduce Vm Reduce Km Increase km Reduce Vm and km 14 Which amino acid residue sequence would mostly likely compose a transmembrane segment of a protein? (2 Points) VLVAVLESFLLLSL GRRHLGFEATFFQ GRKKRRORRRPQ NVFKGNTISOKIS 15 What would be observed if purified IgG was boiled and not reduced and then applied to SDS- PAGE (2 points)
Some bacteria make an enzyme called β-galactosidase that catalyzes the breakdown of the polysaccharide β-galactoside into monomers of galactose. A population of bacteria were found with an amino acid mutation within the active site of β-galactosidase protein, and these bacteria were unable to breakdown β-galactoside polymers. A) How would you identify a population of bacteria with mutant β.galac tosidase in terms of the relative concentration of β-galactoside and galactose in their cells? B.) Explain how the amino acid mutation could...
24. Explain the energetic benefits of making the enzyme active state rather than to the substrate conformation. Also explain how contribute to enzyme action. In other words, how do these weak intera enzyme and the transition state lower the activation energy?) of making the enzyme active site complementary to the transition nation. Also explain how multiple weak interactions words, how do these weak interactions between the 25. 1/F. The pk's of titratable groups of amino acids remain constant irrespective of...
. Gleevec (Imatinib) inhibits protein kinase BCR-ABL, which is
constitutively active in patients with Chronic Myelogenous Leukemia
(CML). The structure of Gleevec is shown below:
a) Based on our discussion in lecture and the structure above,
explain how the “lead” compound structure for Gleevec was designed
and how it led to the development a compound that could bind ABL
with higher affinity than ATP.
b) Using a broad kinase inhibition assay, you discover that
Gleevec inhibits another tyrosine kinase called...
Only answer if you have a background in biochemistry.
Thanks
odon number Normal 506 507 508 509 510 511 Mutant ene (coding strand) ATC ATCTT GGT GTTT gene (coding strand) ATc ATX XXT GGT TCC GTT TCC What effect will this mutation have on the amino acid sequence of the protein encoded by the CFTR A Deletion of a phenylalanine residue with no change in the C-terminus sequence. B. Deletion of a leucine residue with no change in the sequence....
Question 5 Your friend is an amateur mushroom hunter. He accidentally eats an Amanita phalloides "Death Cap" mushroom and becomes very ill. You recall from university that this mushroom is toxic because it contains a aminitin, a noncompetitive inhibitor of RNA polymerase. Which of the following hypothetical treatments (if they were possible) would NOT help your friend? Select all best answers for full credit Injecting more RNA polymerase into his cells. Injecting a drug that binds the enzyme-binding region a...
Please, need help with this problem ! ( Cell Biology )
Thank You
Problem 9 - Panoptes In Ancient Greek mythology, the giant Argus was attributed the nickname "Panoptes" ("all seeing") because he had 100 eyes spread all over his head and body. And our friend Argus Panoptes, herem is afflicted with ubiquitous expression of the eyeless gene Aristotle and molecular biology Nowadays, researchers induce mutations in animal models to better understand mutations in humans In fruit flies, a genetic...
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