Question

What kind of inhibition do you expect inorganic phosphate and L-phenylalanine to display? And why?

Answer 1

The type of inhibition L-phenylalanine display with the enzyme like alkaline phosphatase is uncompetitive inhibition which is due to steric specificity.

L-phenylalanine binds to the enzyme substrate complex rather than to free enzyme. This Enzyme substrate complex is non functional and inhibited and hence Vmax is decreased. This also declines Km value because the reduced uninhibited enzyme substrate complex require low substrate concentration in order to half saturate the enzyme.

Whereas the inorganic phosphate is generally competitive inhibitor as its binding capacity with the enzyme like alkaline phosphatase is same to that of substrates.

It is one of the product formed from the reaction using alkaline phosphatase. It binds reversibly at the substrate binding site of enzyme and prevents substrate to bind. They have same Vmax like substrate but Km is increased.