1. You are given a test tube containing 10 mL of a
solution with 8.4 x 107 cells/mL. You are to
produce a solution that contains less than 100
cells/mL. What dilutions must you perform in order to
arrive at the desired result?
2. You have a microfuge tube containing 1 mL of a solution
with 4.3 x 104 cells/mL and you are to produce
a solution that contains 43 cells/mL. What
dilutions must you perform?
3. You are given a container with 5 mL of a solution
containing 5.1 x 103 cells/mL. You are to
produce a solution that contains
approximately 100 cells/mL.
1. You are given a test tube containing 10 mL of a solution with 8.4 x...
you have a 2 ml solution of CHO cells at a concentration of 5.6 x 10^4 cells/mL and need to produce a solution of 56 cells/mL set up detailed serial dilution given 8mL of 4.1 x10^3 cells/ML and need approximitly 100 cells/mL. set up detailed serial dilution by determining the aliquot to be transfered (v1) from the solution to get 100 cells/mL in a final volume of 10 mL given 10mL cell suspension of NIH cells at 3.8 x10^5 cells/ML...
1. You have been given a tube that contains 2.4 x 109
bacteria/ml. Diagram a complete dilution scheme so that you will
have a plate with 240 colonies on it.
How would you change the scheme you have drawn above so that the
plate had 100 colonies on it?
1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete dilution scheme so that you will have a plate with 240 colonies on it. (3...
1. If you mix a 100 mL liquid solution containing equal parts by moles of benzene, toluene, ethyl-benzene, and o-xylene in a 1 L closed container, what's the partial pressure of toluene in the gas phase of the container? Given the vapor pressure of benzene, toluene, ethyl-benzene, and o-xylene are 12.7 kPa, 3.8 kPa, 0.88 kPa, and 1.27 kPa, respectively. a) 0.95 kPa b) 3.8 kPa c) 4.7 kPa d) 18.65 kPa 2. Given the pKa of acetic acid (CH3COOH)...
You are given a bottle containing 100 mL of a 50X TAE stock solution and are told to make 900 mL of 1X TAE solution. Describe what you would do.
Answer and do the following calculations: 1. Suppose your professor handed you a test tube with 2.0 mL of an E. coli broth culture in it and told you to make a 10–2 dilution of the entire culture. Explain how you would do this. Show your calculations. 2. How would you produce a 10–1 dilution of a 3 mL bacterial sample using the entire 3 mL volume? 3. You have 0.05 mL of an undiluted culture at a concentration of...
You need 100 mL of a 0.00057 M solution for an experiment. You are given 10 mL of a 5.7 M stock solution. What is the best method to get the final volume and concentration you for trial? -It is not possible to reach that concentration with the info presented. -Use a 1.0 mL volumetric pipette and then dilute with water to 100 mL, then repeat serially until the desired concentration is reached. -Use a graduated cylinder to measure 0.1...
You have a tube with 8 ml of splenocytes at a concentration of 9.8 x 106 cells/ml and anything else you may need. The diluting buffer is RPMI/2% FCS. You have at your disposal everything else you may need Question: You need to make five (5) 96 well plates in which each well has 5 x 104 cells in a volume of 100ul. These are going to be used for a cytotoxicity assay. Describe how would you do this, showing...
Given a stock solution of yellow #5 with a concentration of 2.9 x 10-4 M, calculate the concentration of yellow #5 in the 4 prepared solutions (A thru D) assuming you perform these dilutions exactly as required in the procedure of this lab. 50 mL Beaker Dye stock solution 1:1 Ethanol/Water solvent A 1.00 mL 9.00 mL B 3.00 mL 7.00 mL C 5.00 mL 5.00 mL D 7.00 mL 3.00 mL
A tube containing 8.00 mL of 0.250 M copper (II) sulfate contains 2.00 x 10^-3 mole of copper (II) sulfate. how many grams of solid copper (II) sulfate would this be equivalent to? using dimensional analysis
you perform three 1:3 dilutions from a stock solution. You then remove 100 microliters from your last test tube and plate on a nutrient agar plate. You count 167 colonies post incubation. What was the concentration of the original culture (CFU/mL)? a45090 b4509 c6.9 d16700