1. You have been given a tube that contains 2.4 x 109 bacteria/ml. Diagram a complete dilution scheme so that you will have a plate with 240 colonies on it.
How would you change the scheme you have drawn above so that the plate had 100 colonies on it?
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1. You have been given a tube that contains 2.4 x 109
bacteria/ml. Diagram a complete...
A. You have been given a tube of E. coli. You are asked to make 1...
A. You have been given a tube of E. coli. You are asked to make 1 mL total volume of 10-1 dilution of the bacterial culture. Explain how you would do this. Show all necessary calculations. ____ ml cells + _____ ml water = 1 ml (total volume) B. Next, you were asked to make a 10-2 dilution of the bacterial sample. Explain how you would perform this. Show all necessary calculations. You have bacteria at a concentration of 2...
You have a tube with 5ml of infected blood that has a concentration of bacteria at...
You have a tube with 5ml of infected blood that has a concentration of bacteria at 10(8) cells per ml. You wish to make a serial dilution of the blood so when you take 100 ul of the last dilution and spread it on a Petri dish you will have between 30-300 colonies growing on the plate. You have plenty of empty 1.5 ml microfuge tubes, a bottle of sterile water, a p1000 pipettor (100-1000ul) and tips, for the dilutions,...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1...
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
data given Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml...
data given
Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml I ml Tube 11 Tube 21 Tube 3 Tube 4 Il ml 1 ml ml No of bacterial Colonies TNTC TNTC 290 35 Using the Pour-Plate Method What was the purpose of this lab exercise? Complete the following table with your observations: Dilution factor Comments Number of bacteria per 1 ml of original milk Number of colonies on the plate 10 100 1,000 10,000...
4. Calculate the amount of bacteria in the original sample from the bacterial dilution below (3pt)...
4. Calculate the amount of bacteria in the original sample from the bacterial dilution below (3pt) p.lm 0.1ml> .Im! Sample 0.9ml 9.9ml 0.9ml 10.1ml 10.1ml 10.1ml TNTC 544 5. You have been given 10 ul (0.010ml) of a bacterial stock that contains 7 x 1010 CFU/ml. Diagram a complete dilution scheme that will give you a plate with 100 CFU/ml on it. Be sure to include all steps and remember that you have only 10 ul as an initial sample...
You have a stock of an E. coli cell culture of an unknown concentration. You want...
You have a stock of an E. coli cell culture of an unknown concentration. You want to set up a serial dilution experiment so that you can count the viable colonies and determine the concentration of the stock in cells/ml. In tube A, you add 1 ml of the stock to 999 ml of media. In tube B, you add 1 ml of the material from tube A to 24 ml of media. In tube C, you add 50 microliters...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates,...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
14. Analyze the following serial dilution experiment and answer the questions, each (a and b) worth...
14. Analyze the following serial dilution experiment and answer the questions, each (a and b) worth 10 pts. Given: One ml of the original bacterial culture (tube 0) was added to experiment tube 1, containing 9 ml of media. 7 more experiment tubes were serially diluted in order, the same way, for a total of 8. Each originally contained 9 ml of media, and each received 1 ml from the tube number preceeding it. Two drops from tube #8 were...
1. You are given a test tube containing 10 mL of a solution with 8.4 x...
1. You are given a test tube containing 10 mL of a solution with 8.4 x 107 cells/mL. You are to produce a solution that contains less than 100 cells/mL. What dilutions must you perform in order to arrive at the desired result? 2. You have a microfuge tube containing 1 mL of a solution with 4.3 x 104 cells/mL and you are to produce a solution that contains 43 cells/mL. What dilutions must you perform? 3. You are given...
You are given a fresh meat sample containing 5.6 times 10^5 bacteria/g. Diagram how you would...
You are given a fresh meat sample containing 5.6 times 10^5 bacteria/g. Diagram how you would dilute and plate this sample so that you that the resulting plates have colony numbers in a countable range. Predict how many colonies would be present on each plate.
Dilution Practice Problem #5 You are given the following bacterial dilution scheme: ml 1 ml 1 ml 1 ml 1 ml CODILE 9ml 9ml 9ml 0.1 ml 0.1 ml colonies) (colonies colonies) Individual tube dilutions: BCD Dilution Factor: Total tube dilutions) IDE AB Plated dilution factor 1. Which plate is the countable plate? (30-300 countable range) 2. Calculate the titer of the original culture in cfu/ml.
data given
Procedure for Serial Dilutions: Experimental Data is given II ml 1 ml ml ml I ml Tube 11 Tube 21 Tube 3 Tube 4 Il ml 1 ml ml No of bacterial Colonies TNTC TNTC 290 35 Using the Pour-Plate Method What was the purpose of this lab exercise? Complete the following table with your observations: Dilution factor Comments Number of bacteria per 1 ml of original milk Number of colonies on the plate 10 100 1,000 10,000...
4. Calculate the amount of bacteria in the original sample from the bacterial dilution below (3pt) p.lm 0.1ml> .Im! Sample 0.9ml 9.9ml 0.9ml 10.1ml 10.1ml 10.1ml TNTC 544 5. You have been given 10 ul (0.010ml) of a bacterial stock that contains 7 x 1010 CFU/ml. Diagram a complete dilution scheme that will give you a plate with 100 CFU/ml on it. Be sure to include all steps and remember that you have only 10 ul as an initial sample...
2. A series of 1 ml samples from tube #4 of the dilution s eries below are spread on agar plates, resulting in the growth of an average of 68 colonies per plate. What is the concentration of colony-forming units (cfu/mi) present in the original culture? 0.1 ml 0.1 ml 1 ml 1 ml 100 ml 9.9 ml 9.9 ml 9 ml 9 ml 2 4 3. The culture flask at left contains a total of 3.2 x 10s cfu....
14. Analyze the following serial dilution experiment and answer the questions, each (a and b) worth 10 pts. Given: One ml of the original bacterial culture (tube 0) was added to experiment tube 1, containing 9 ml of media. 7 more experiment tubes were serially diluted in order, the same way, for a total of 8. Each originally contained 9 ml of media, and each received 1 ml from the tube number preceeding it. Two drops from tube #8 were...
You are given a fresh meat sample containing 5.6 times 10^5 bacteria/g. Diagram how you would dilute and plate this sample so that you that the resulting plates have colony numbers in a countable range. Predict how many colonies would be present on each plate.
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