Question

please help (no need to explain just give me the answer please) ==> 1) The in vitro translation system Nirenburg & Matthaei used to determine the first “word” of the genetic code technically SHOULD NOT have worked because: a) The cell extract technically didn’t contain living ribosomes b) The synthetic polyU RNA added did not contain a ribosome binding site or start codon c) RNA molecules break down too quickly to be effectively translated in a cell-free extract d) The tobacco mosaic virus RNA used in the experiment is the “nonsense” = template strand, not the coding strand

==> 18) 18) When the large ribosomal subunit first binds to the small ribosomal subunit to begin translation, what site is the methionine initiator tRNA located in? a) P site b) A site c) E site d) None of the above, the initiator tRNA exits the ribosome immediately before assembly of the 2 subunits

Answer 1

Ans:

1) The in vitro translation system Nirenburg & Matthaei used to determine the first “word” of the genetic code technically SHOULD NOT have worked because:

b) The synthetic polyU RNA added did not contain a ribosome binding site or start codon.

2) When the large ribosomal subunit first binds to the small ribosomal subunit to begin translation, what site is the methionine initiator tRNA located in?

  a) P site.