Question

1. A ____________________ prefers to grow at temperatures between 25-40⁰C.

   	A.	barophile
   	B.	halophile
   	C.	mesophile
   	D.	psychrophile
   	E.	all of the above
   	F.	none of the above

2. In the aerobic respiration experiment, ________ bacteria will show a small amount of growth towards the top of the media; however, it is not growing at the surface, so no pellicle forms; the red layer of indicator is still present, and there is only a small amount of bacterial growth in the red layer.

	A.	aerotolerant anaerobe
	B.	facultative anaerobe
	C.	microaerophile
	D.	obligate aerobe
	E.	all of the above
	F.	none of the above

3. ______________________ media is used to encourage the growth of highly fastidious organisms, and it has been supplemented with highly nutritious materials in order to do so.

	A.	differential
	B.	distinctive
	C.	enriched
	D.	selective
	E.	all of the above
	F.	none of the above

4. The _________ is the temperature that cells divide the quickest at.

	A.	maximum growth temperature
	B.	medial growth temperature
	C.	minimum growth temperature
	D.	optimum growth temperature
	E.	all of the above
	F.	none of the above

5. Fermentation media doesn’t include ______________________________.

1. 	A.	a pH indicator to show the presence of acid
   	B.	a specific carbohydrate to determine fermentation capability
   	C.	growth inhibitory substances
   	D.	standard nutrient broth ingredients
   	E.	all of the above
   	F.	none of the above

6. When a dilute culture is mixed into molten agar and placed into a petri dish, this is a type of subculturing termed _______________.

1. 	A.	pour plate method
   	B.	serial dilutions
   	C.	spread plate method
   	D.	streak plate method
   	E.	all of the above
   	F.	none of the above

7. A bacterium that is rod-shaped is called ________, and one that is spherical is called _________.

1. 	A.	bacillus, cocci
   	B.	cocci, bacillus
   	C.	pleomorphic, spirochete
   	D.	spirochete, pleomorphic
   	E.	all of the above
   	F.	none of the above

8. A common stain used to classify and differentiate bacteria is the __________; this test is based on differences in the chemical composition of bacterial cell walls. A positive test occurs when a thick layer of peptidoglycan is found on the outside of the cell membrane.

1. 	A.	endospore stain
   	B.	Gram stain
   	C.	negative stain
   	D.	simple stain
   	E.	all of the above
   	F.	none of the above

9. __________ isn’t a possible result in a Fermentation assay.

	A.	a change in color from red to yellow
	B.	cloudiness or debris in the test tube
	C.	no discernible change in the appearance of the media
	D.	the presence of a bubble in the Durham tube
	E.	all of the above
	F.	none of the above

Answer 1

1) Option 'c' is the correct answer.

Mesophiles are the organisms that grow best in medial temperature range between 25-40^oC. Pyschrophiles grow at colder temperatures often ranging in the subzero range, while thermophiles prefer higher temperatures in the range of 60-80^oC. Barophiles prefer high-pressure environment for optimum growth, while halophiles live in high-salt concentration environments.