Question

1) The DNA template is read in the ______ direction, and the new strand is synthesized in the ____ direction. A) 5' to 3' ; 3' to 5' B) 5' to 3' ; 5' to 3' C) 3' to 5' ; 5' to 3' D) 3' to 5' ; 3' to 5'

2) If a sequence of DNA is 5' AATTGCCGT 3', the complementary strand would be A) 3' AAAACGCCA 5' B) 3' TTAACGGCT 5' C) 5' ACGGCAATT 3' D) 5' TTAACGGCA 3'

3) What is the basis for the difference in how the leading and lagging strands of DNA molecules are synthesized? A) The origins of replication occur only at the 5' end. B) Helicases and single-strand binding proteins work at the 5' end. C) DNA polymerase can join new nucleotides only to the 3' end of a growing strand. D) DNA ligase works only in the 3' →5' direction. E) Polymerase can work on only one strand at a time.

4) When comparing the leading and lagging strands in DNA replication, A) the leading strand is synthesized in the 3' to 5' direction, and the lagging strand is synthesized in the 5' to 3' direction. B) the leading strand is synthesized continuously, whereas the lagging strand is synthesized in short fragments that are ultimately covalently bonded together by ligase. C) More Okazaki fragments are found in the leading strand and the lagging strand. D) both the leading and lagging strand are synthesized in the direction of the replication fork

Answer 1

1.
The DNA template is read in 3' to 5' direction and the new strand is synthesized in the 5' to 3' direction.
Option c is the correct answer

2.
Given the sequence of DNA is 5' AATTGCCGT 3'
The complementary strand is 3' TTAACGGCA 5'
Option D is the answer

3.
In the leading strand, the nucleotides are added in the 3' growing end of the strand.
The DNA polymerase can perform this action only in 3' end but not to 5' end.
Option C is the answer

4.
The leading strand is continuously synthesized hence it is a continuous fragment.
The lagging strand is synthesized in short fragments. These lagging strand fragments
are later joined by DNA ligase.
Option B is the answer