A.) For the given problem, a wavelength of 560 nm corresponds to the absorption maxima of Amylopectin present in starch. The absorptivity values resulting from this measurement can be used for the quantification of amylopectin and thus starch indirectly.
B.) Iodine binds with amylose and amylopectin (in starch) to produce a blue color which is measured spectrophotometrically for the quantification of starch. However, before proceeding with the actual measurement, it is important to rule out background contamination of starch present in either iodine or water (used as a solvent). Thus, to subtract the background contamination values of absorption, we use water+iodine as a blank for the spectrophotometer.
А) Why is a spectrophotometer used at a wavelength of 560nm? В) Why is water +...