Question

tRNAs that contain introns do not have closely related intron sequences. How is it that all tRNAs are spliced via the same mechanism?

Answer 1

Answer:             This question i have to write pointswise,because it's easy to understand you....

• Transfer RNA splicing is a special form of splicing that involves a different mechanism as compared to spliceosomal or self-splicing pathways.
• In the yeast, a tRNA splicing endonuclease tetramer (composed of TSEN2, TSEN15, TSEN34, and TSEN54) cleaves the pre-tRNA at two sites.
• This cleavage generates two fragments:
• i) The 5'-half terminating at a 2', 3'-cyclic phosphodiester group
• ii) The 3'-half terminating at a 5-OH group along with the intron
• A yeast tRNA phosphodiesterase cleaves the cyclic phosphodiester group to generate 2'-phosphorylated 3'-end.
• A yeast tRNA kinase phosphorylates the 5'-OH group using ATP.
• A yeast tRNA ligase adds the AMP group to the 5'-end of the 3'-half and joins the 2 halves together.

• A phosphotransferase (NAD-dependent) catalyzes the removal of 2'-Phosphate group

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