Question

Antibiotic resistant bacteria are becoming more and more prevalent, leaving us in desperate need of novel antimicrobial compounds to replace the drugs that are now obsolete in our hospitals and medical clinics. A high throughput screen of 700,000 compound library has allowed you to identify one molecule capable of killing extensively drug resistant tuberculosis at concentrations 1000X lower than currently used therapeutics. The only problem is, you don't know the molecular target. Develop an experimental strategy to identify the molecular target of your new antibiotic.

Answer 1

We have the molecule capable of killing extensively drug resistant tuberculosis. We need to develop an experimental strategy to identify the molecular target of your new antibiotic.

Following steps can be done :

1. Collect proteomics data of tuberculosis and collect structural data of all proteins in it.
2. Then perform molecular docking with the molecule you already have.
3. This can give the molecular target of our new antibiotic.