Question

An inhibitor is created for an enzyme that causes cancer. Kinetics data (Km and Vmax) is generated and listed below.

With this data, what conclusions can you say about the inhibitor?

Enzyme with Inhibitor: (Km= 2.3 M & Vmax= 1.17 s^-1)

Enzyme without inhibitor: (Km= 2.5 M & Vmax= 4.49 s^-1)

Answer 1

Answer:- Enzyme with Inhibitor: (Km2.3 M & Vmax= 1.17 s^-1)
Enzyme without inhibitor: (Km= 2.5 M & Vmax= 4.49 s^-1)
For this inhibitor both Vmax and Km values are decreased. So it is a uncompetitive inhibitor.This inhibitor binds to enzyme-substrate complex; but not to the free enzyme.