In Exercise 7.2, timed samples were mixed with glutaraldehyde before microscopic examination. Wha...
In Exercise 7.2, timed samples were mixed with glutaraldehyde before microscopic examination. What was the purpose of this step? One of the available treatment conditions was a T. vorax growth medium adjusted to pH 5. For what purpose was this solution provided?
In Exercise 7.2, timed samples were mixed with glutaraldehyde before microscopic examination. What was the purpose of this step?
Homework Answers
- Glutaraldehyde is incomparable in its capacity to conserve cell ultrastructure. It is due to the introduction of irreversible intra-and intermolecular cross-links into cellular proteins by the dialdehyde. Glutaraldehyde is very efficient in stabilizing surface as well as intracellular structures for microscopic examination.
- T. vorax growth medium adjusted to pH 5 in order to prevent digestion of the India ink particles in the phagolysosome as phagocytosis decreases with decrease in pH. Literature review demonstrates that phagocytosis is lowest at pH 5 while it is equal at pH 6 and pH 7.
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